ELEISA KIT yeTrypsin
Tsanangudzo
Recombinant Trypsin inowanzoshandiswa mukugadzira biopharmaceutical-panguva yekugadzirira masero kana kugadziridzwa uye activation yezvigadzirwa.Trypsin inoisa njodzi yekuchengetedza saka inofanirwa kubviswa isati yaburitswa chigadzirwa.Iyi Sandwich Kit ndeyekuyera kuyerwa kweResidual Trypsin mune cell tsika supernatant uye mamwe maitiro mukugadzira biopharmaceutical kana Trypsin yashandiswa.
Iyi kit ndeye Enzyme-Yakabatana Immunosorbent Assay (ELISA).Iyo ndiro yakambovharwa nePorcine trypsin antibody.Trypsin iripo mumucherechedzo inowedzerwa uye inosungirirwa kune masoja ekudzivirira chirwere akaputirwa pamatsime.Uyezve biotinylated Porcine trypsin antibody inowedzerwa uye inosunga kune trypsin mumuenzaniso.Mushure mekugeza, HRP-Streptavidin inowedzerwa uye inosunga kune Biotinylated trypsin antibody.Mushure mekuita incubation unbound HRP-Streptavidin inogezwa.Ipapo TMB substrate mhinduro inowedzerwa uye yakagadziridzwa neHRP kugadzira yebhuruu ruvara chigadzirwa chakashanduka kuita yero mushure mekuwedzera acidic stop solution.Kuwanda kweyero kunoenzanirana nehuwandu hwechinangwa chetrypsin
muenzaniso wakatorwa mundiro.Iyo absorbance inoyerwa pa450 nm.
Kemikari Mamiriro
Tsanangudzo
| Test Items | Zvinotsanangurwa |
| Chitarisiko | Yakazara kurongedza uye hapana mvura inodonha |
| Muganho wakaderera wekuonekwa | 0.003 ng/mL |
| Muganho wepasi wehuwandu | 0.039 ng/mL |
| Precision | Intra assay CV≤10% |
Kutakura uye kuchengetedza
Zvekufambisa:Ambient
Kuchengeta:Inogona kuchengetwa pa -25 ~ -15 ° C muhupenyu hwesherufu, 2-8 ° C kune imwe nzira yekuedza
Inokurudzirwa edzazve Hupenyu:1 gore
