BsaI Restriction Endonuclease
Tsanangudzo
Iyo BsaI restriction enzyme imhando II restriction endonuclease yakabva muprotein recombinant encoded neBspQI gene muBacillus sphaericus inoratidzwa neE.coli.Kurambidza endonucleases anoshandiswa zvakanyanya mukugadzira gene, cloning uye mamwe minda.Enzayemu iyi inokurumidza kucheka DNA kuti iite gene mutsara Bsa I, IIs restriction endonuclease restriction endonuclease, inotorwa kubva kune recombinant E. coli strain inotakura yakaumbwa uye yakagadziridzwa BsaI gene kubva kuBacillus stearothermophilus.ayo ane hunhu hwakafanana nema enzymes ekuzvarwa uye kuderedzwa kwenyeredzi kuita.Kuzivikanwa kwayo kutevedzana uye cleavage nzvimbo ndeaya anotevera:
5'······GGTC(N)······· ··3'
3'······CCAGAG(NNNNN)·······5'
Kemikari Mamiriro
Product features
• The restriction endonuclease ine high enzymatic activity uye inopedza digestion nekukurumidza.
• Yakaderera isiri-yakananga endonuclease basa kuti uve nechokwadi chekucheka "scalpel" chaiyo.
• Hapana BSA hurongwa, kuderedza kupisa tsime kusvibiswa.
Tsanangudzo
| Test Items | Zvinotsanangurwa |
| Enzyme Chiitiko | 20 U/µL |
| Kuchena(SDS-PEJI) | ≥95% |
| Rnase | Hapana chaonekwa |
| Kwete-Yakananga DNase Chiitiko | Hazvina kuonekwa |
| Star Chiitiko | Hazvina kuonekwa |
| Ligation uye Recuting | Mushure mekugaya, zvidimbu zveDNA zvinogona kubatanidzwa.Uye izvi zvimedu zvine ligated zvinogona kuchekwa neBsal. |
| Endotoxins(LAL-test) | ≤10 EU/mg |
| E.Coli DNA | ≤1 Kopi/2U |
Kudzora kwehutano
Kuchena ≥ 95%, Hapana DNase, RNase basa, Host DNA yakasara ≤ 100pg/mg, mapuroteni anosara ≤ -50ppm, endotoxin yakasara ≤ 10EU/mg, Hapana basa reprotease, sterile, isina mycoplasma
Application
Digest the plasmid kugadzirira linearized DNA fragment pakupera kwePoly (A/T/C/G);
Linearize plasmid template isati yanyorwa in vitro;
Restriction digest;
Chikamu Tsanangudzo
Imwe yuniti inotsanangurwa sehuwandu hwe enzyme inodiwa kugaya 1 µg yeInternal control DNA muawa imwe pa37 ° C muhuwandu hwekuita vhoriyamu ye50 µL.
Kutakura uye kuchengetedza
Zvekufambisa:Inotumirwa pasi -15°C
Kuchengeta:Chengetedza pa -25~-15°C
Inokurudzirwa edzazve Hupenyu:1 gore
