Alkaline Phosphatase (ALP)
Tsanangudzo
Alkaline Phosphatase inotorwa kubva kune recombinant E. coli strain inotakura jena reTAB5.Iyo enzyme inokonzera dephosphorylation ye5' uye 3' magumo eDNA uye RNA phosphomonoesters.Zvakare, iyo hydrolyses ribose, pamwe ne deoxyribonucleoside triphosphates (NTPs uye dNTPs).TAB5 Alkaline Phosphatase inoshanda pane 5' yakasimudzwa, 5' yakadzokororwa uye yakagomara magumo.Iyo Phosphatase inogona kushandiswa mune akawanda mamorekuru biology application, senge cloning kana probe end labeling kubvisa phosphorylated magumo eDNA kana RNA.Mukuedza kwecloning, dephosphorylation inodzivirira iyo linearized plasmid DNA kubva pakuzvibatanidza.Inogona zvakare kudzikisa zvisina kubatanidzwa dNTPs muPCR maitiro kugadzirira template yeDNA kutevedzana.Iyo enzyme inogadziriswa zvachose uye isingachinjiki nekudziya pa70 ° C kwemaminetsi mashanu, zvichibva zvaita kuti kubviswa kwephosphatase kusati kwasungwa kana kupedzisa kunyora zvisina kufanira.
Kemikari Mamiriro
Tsanangudzo
| Test Items | Zvinotsanangurwa |
| Enzyme Chiitiko | 5U/μL |
| Kuchena | ≥ 95% |
| Endonuclease Chiitiko | Haioneki |
| Exonuclease Chiitiko | Haioneki |
| Nicking Chiitiko | Haioneki |
| RNase Chiitiko | Haioneki |
| E.coli DNA | ≤1kopi/5U |
| Endotoxin | LAL-Muedzo, ≤ 10EU/mg |
Kutakura uye kuchengetedza
Zvekufambisa:Ice packs
Kuchengeta:Chengetedza pa -25~-15 ° C (usadzokorore kutonhora uye kunyunguduka)
Inokurudzirwa edzazve Hupenyu:2 gore
