T4 RNA ligase
Tsanangudzo
Ichi chigadzirwa iATP-inotsamira T4 RNA ligase (T4 RNA Ligase I) inoratidzirwa zvakare neE. coli.Iyi enzyme inokonzera kuumbwa kwephosphodiester zvisungo pakati pe oligonucleotides, single-stranded RNA uye DNA intermolecular / intra-molecular 5'-PO4 uye 3'-OH.
Chikamu Tsanangudzo
Chimwe chikamu chinotsanangurwa sehuwandu hweenzyme inodiwa kushandura 1 nanomole ye 5'-[³²P] rA16 kuita phosphatase-resistant fomu mumaminitsi makumi matatu pa37 ° C.
Tsanangudzo
| Test Items | Zvinotsanangurwa |
| Enzyme Chiitiko | 10 U/μL |
| Mapuroteni Basa | ≥ 95% |
| Nickase | Haioneki |
| RNase Chiitiko | Haioneki |
| Ecoli gDNA | ≤1kopi/50U |
| Endotoxin | LCL-Test,≤10EU/mg |
Kutakura uye kuchengetedza
Zvekufambisa:Dry ice
Kuchengeta:Chengetedza pa -25 ~ -15 ° C (usadzokorore kutonhora uye kunyunguduka)
Inokurudzirwa edzazve Hupenyu:2 gore
zvinoenderana zvigadzirwa
Nyora meseji yako pano ugotitumira
