Recombinant trypsin
Advantage
1.No mhuka yakabva
2.Kuchena kwepamusoro
3.Better process control
4.Stable quality
Tsanangudzo
Trypsin inonyanya kutsemura C-terminal peptide zvisungo zvelysine uye arginine, izvo zvinogona kukanganisa intercellular kusunga mapuroteni.Kutevedzana kweamino acid yetrypsin inosanganiswa inogadzirwa neHzymes Biotech yakafanana neiyo porcine pancreas-derived trypsin, uye inogadzirwa nerecombinant Escherichia coli kutaura.Inogona kutsiva yechinyakare kudhirowa kwetrypsin kwesero digestion mumajekiseni, stem masero, immune cell therapy, kuongororwa kwezvinodhaka, masoja ekudzivirira chirwere uye mimwe minda.Aprotinin, soybean trypsin inhibitor, nezvimwewo zvinogona kurambidza zvakajeka kuita kwetrypsin.
Recombinant trypsin ine zvimiro zvakafanana neyakatorwa trypsin.Ichi chigadzirwa chinogadzirwa muGMP giredhi workshop.Hapana mhuka mavambo mbishi zvinhu zvinoshandiswa mukuvirisa, kucheneswa uye kwekupedzisira zvisanganiswa.Panguva imwecheteyo, maitiro ekugadzira uye maitiro ekugadzirisa maitiro anosimbiswa, zvichiita kuti pave nehupamhi hwekuenderana pakati pemabheti.
Kemikari Mamiriro
Chikamu Tsanangudzo
25 ℃, pH 7.6, 3.2ml reaction solution (1cm chiedza nzira), imwe trypsin unit (U) yakatsanangurwa se
kuwedzera kwe0.003 mukunyura ukoshi pa253nm ne enzymatic hydrolysis yeBAEE paminiti.
Kushandiswa Kwechigadzirwa
Nezvesero tsika
• Tissue block digestion, yekutanga masero ekutora.
• Kugayiwa kwemaseru anobatirira.
• Sero tsika kubudikidza microcarrier nzira.
• Kuchera zvinyoro masero.
• Immune cell therapy, nezvimwewo.
Nezve recombinant protein
• Recombinant insulin kugadzirwa.
• Kurongeka kweprotein, peptide mepu.
• Yakananga maitiro eproteolytic akadai seproteomics research
Tsanangudzo
| Test Items | Zvinotsanangurwa |
| Chitarisiko | Upfu huchena kana husina kuchena |
| Solubility (10mg/ml) | Yakajeka mhinduro isina ruvara |
| Kuchena | Β-trypsin ≥70%, α-trypsin ≤ 20% |
| Chaiyo Chiitiko(Upfu) | ≥2500U/mg |
| E.Coli host protein residue | ≤0.01% |
| Endotoxins(LAL-Test) | ≤20 EU/mg |
Kutakura uye kuchengetedza
Zvekufambisa:Inotumirwa pasi pe2-8 °C
Kuchengeta:Chengetedza pa -2-8 ° C, mushure mekunyungudika ne 1mM hcl kana 50nM HAC, inofanira kuchengetwa pasi -25 ~ -15 °C.
Inokurudzirwa edzazve Hupenyu:2 gore
