Deoxyribonuclease I (Dnase I)
Tsanangudzo
DNase I (Deoxyribonuclease I) inonzi endodeoxyribonuclease inokwanisa kugaya DNA imwe kana ine tambo mbiri.Inoziva uye inotsemura mabhondi ephosphodiester kugadzira monodeoxynucleotides kana oligodeoxynucleotides imwe kana kaviri-yakasungwa nemapoka ephosphate pa5'-terminal uye hydroxyl pa3'-terminal.Chiitiko cheDNase I chinoenderana neCa 2+ uye chinogona kuitwa ne divalent metal ions seMn 2+ uye Zn 2+.5 mM Ca 2+ inodzivirira iyo enzyme kubva kuhydrolysis.Pamberi peMg 2+, enzyme yaigona kuona nekutsemura chero nzvimbo pane chero tambo yeDNA.Pamberi peMn 2+, tambo mbiri dzeDNA dzinogona kuzivikanwa panguva imwe chete uye dzakatsemurwa panzvimbo inenge imwe chete kuti iite zvimedu zveDNA zvakati sandara kana kuti zvimedu zveDNA zvinonamira zvine 1-2 nucleotides.
Kemikari Mamiriro
Chikamu Tsanangudzo
Chimwe chikamu chinotsanangurwa sehuwandu hweenzayimu inozosvibisa zvachose 1 µg yepBR322 DNA mumaminetsi gumi pa37°C.
Tsanangudzo
| Test Items | Zvinotsanangurwa |
| Kuchena (SDS-PEJI) | ≥ 95% |
| Rnase Chiitiko | No Degradation |
| gDNA Kusvibiswa | ≤ 1 kopi/μL |
Kutakura uye kuchengetedza
Zvekufambisa:Inotumirwa pasi pe0 °C
Kuchengeta:Chengetedza pa -25~-15°C
Inokurudzirwa edzazve Hupenyu:2 gore
