Virus DNA/RNA Extraction Kit
Iyo kit (HC1009B) inogona kukurumidza kubvisa yakakwira-kuchena viral nucleic acids (DNA/RNA) kubva kune akasiyana emvura samples seropa, serum, plasma, uye swab yekuwachisa mvura, ichigonesa yakakwirira-kuburikidza kugadziridzwa kwemasampuli akafanana.Iyo kiti inoshandisa yakasarudzika yakamisikidzwa superparamagnetic silicon-yakavakirwa magineti bead.Mune yakasarudzika buffer sisitimu, nucleic acids panzvimbo yemapuroteni uye kumwe kusvibiswa kunoshambadzirwa nehydrogen bond uye electrostatic binding.Magineti mabedhi ane adsorbed nucleic acids anogezwa kubvisa mapuroteni asara nemunyu.Paunenge uchishandisa pasi-munyu-buffer, nucleic acids inosunungurwa kubva kune magnetic beads, kuitira kuti uwane chinangwa chekukurumidza kupatsanurwa uye kucheneswa kwe nucleic acids.Iyo yese mashandiro ekuita iri nyore, inokurumidza, yakachengeteka uye inoshanda, uye yakawanikwa nucleic acids inogona kushandiswa zvakananga kune yakadzika bvunzo senge reverse transcription, PCR, qPCR, RT-PCR, RT-qPCR, inotevera-chizvarwa kutevedzana, biochip kuongorora, etc.
Kuchengetedza mamiriro
Chengetedza pa15 ~ 25 ℃, uye takura patembiricha yekamuri.
Applications
Ropa, serum, plasma, swab eluent, tishu homogenate nezvimwe.
Experiment Process
1. Muenzaniso processing
1.1 Kumavhairasi ari mumasampuli emvura akadai seropa, serum, uye plasma: 300μL ye supernatant inoshandiswa pakubvisa.
2.2 Yemasampu e swab: Isa masampula e swab mumachubhu ekuenzanisira ane mushonga wekuchengetedza, vortex ye1 min, uye tora 300μL supernatant kuti ibvise.
1.3 Kune mavhairasi ari mumatishu homogenates, tissuesoak solutions, uye ezvakatipoteredza samples: Mira masampuli kwe5 -10 min, uye tora 300μL ye supernatant kuti ubvise.
2. Kugadzirira kwe prepackaged reagent
Bvisa pre-package reagents kubva pakiti, invert uye sanganisa kakawanda kuti umise zvakare magineti mabhedhi.Zvinyoro zunza ndiro kuti reagents uye magineti beads kunyura pasi petsime.Ndokumbira usimbise kutungamira kwendiro uye unyatso kubvarura kuvhara aluminium foil.
Dzivisa kudedera paunenge uchibvarura firimu rekuvhara kudzivirira kuti mvura isadeuka.
3. Kushanda kwe otomatikiatic chiridzwa
3.1 Wedzera 300μL yemuenzaniso kumatsime muColumns 1 kana 7 ye96 yakadzika tsime ndiro (teerera kune inoshanda inoshanda zvakanaka chinzvimbo).Iyo yekuisa vhoriyamu yesampuli inoenderana ne100-400 μL.
3.2 Isa 96-tsime rakadzika tsime ndiro munucleic acids extractor.Isa pamagineti bar sleeve, uye ita shuwa kuti inoputira magineti tsvimbo.
3.3 Seta chirongwa ichi sechinotevera chekutora otomatiki:
3.4 Mushure mekudhirowa, chinjisa eluent kubva kuColumns 6 kana 12 ye96 yakadzika tsime ndiro (teerera kune inoshanda inoshanda zvakanaka chinzvimbo) kune yakachena Nuclease-isina centrifuge chubhu.Kana ukasaishandisa nekukasika, ndapota chengetedza zvigadzirwa pa -20 ℃.
Notes
Yekushandisa tsvakurudzo chete.Kwete kushandiswa mukuongorora maitiro.
1. Chigadzirwa chakabviswa DNA/RNA.Kunyanya kutarisisa kunofanirwa kubhadharwa kudzivirira kuderedzwa kweRNA neRNase panguva yekushanda.Midziyo nemasamplers anoshandiswa anofanira kutsaurirwa.Ese machubhu nemapipetti matipi anofanirwa kuvharirwa uye DNase/RNase-isina.Vashandi vanofanirwa kupfeka magurovhosi asina hupfu nemasiki.
2. Ndapota verenga bhuku remirayiridzo zvakanyatsonaka usati washandisa, uye shandisa zvakanyatsoenderana nebhuku rekuraira.Sample processing inofanirwa kuitwa mubhenji rakachena kana biological kuchengetedza kabati.
3. Iyo otomatiki nucleic acid extraction system inofanirwa kuvharirwa neUV kwemaminetsi makumi matatu isati yatanga uye mushure mekushandisa.
4. Panogona kunge paine twumagineti mabedhi asara munzeve mushure mekubviswa, saka dzivisa kushuvira magineti bead.Kana magineti mabedhi achishuvira, anogona kubviswa negineti kumira.
5. Kana pasina mirairo yakakosha yezvikwata zvakasiyana-siyana zve reagents, tapota usazvisanganisa, uye ona kuti makiti anoshandiswa mukati menguva yechokwadi.
6. Rasa zvakanaka masampuli ese uye reagent, nyatso pukuta pasi uye kuuraya utachiona hwese hwebasa ne75% ethanol.
