Viral DNA/RNA Extraction Kit
Iyi kit inokodzera kukurumidza kutorwa kwepamusoro-kuchena hutachiona hweDNA / RNA kubva kumasampuli akadai nasopharyngeal swabs, zvakatipoteredza swabs, cell tsika supernatants, uye tissue homogenate supernatants.Iyo kit yakavakirwa pa silica membrane yekuchenesa tekinoroji inobvisa kudiwa kwekushandisa phenol/chloroform organic zvinonyungudutsa kana nguva inopedza doro kunaya kuti ibvise hutachiona DNA/RNA yemhando yepamusoro.Iwo nucleic acids anowanikwa haana tsvina uye akagadzirira kushandiswa mukuyedza kwakadzika senge reverse transcription, PCR, RT-PCR, chaiyo-nguva PCR, inotevera-chizvarwa sequencing (NGS), uye Northern blot.
Kuchengetedza mamiriro
Chengetedza pa15 ~ 25 ℃, uye kutakura patembiricha yekamuri
Zvikamu
| Zvikamu | 100RXNS |
| Buffer VL | 50 ml |
| Buffer RW | 120 ml |
| RNase-yemahara ddH2 O | 6 ml |
| FastPure RNA Columns | 100 |
| Machubhu ekuunganidza (2ml) | 100 |
| RNase-yemahara Kuunganidza Machubhu(1 .5ml) | 100 |
Buffer VL:Ipa nharaunda ye lysis uye kusunga.
Buffer RW:Bvisa mapuroteni akasara uye mamwe matsvina.
RNase-yemahara ddH2O:Elute DNA/RNA kubva kune membrane mune inotenderera column.
FastPure RNA Columns:Kunyanya adsorb DNA / RNA.
Machubhu ekuunganidza 2 ml:Unganidza filtrate.
RNase-isina Kuunganidza Machubhu 1.5 ml:Unganidza DNA/RNA.
Applications
Nasopharyngeal swabs, zvakatipoteredza swabs, cell tsika supernatants, uye tishu homogenate supernatants.
Kuzvigadzirira Materials
RNase-isina pipette mazano, 1.5 ml RNase-isina centrifuge machubhu, centrifuge, vortex mixer, uye pipettes.
Experiment Process
Ita ese anotevera matanho mune biosafety cabinet.
1. Wedzera 200 μl yemuenzaniso kune RNase-isina centrifuge chubhu (ita nePBS kana 0.9% NaCl kana sampuli isina kukwana), wedzera 500 μl yeBuffer VL, sanganisa zvakanaka nevortexing kwe15 - 30 sec, uye centrifuge. muchidimbu kuunganidza musanganiswa pasi pechubhu.
2. Isa FastPure RNA Columns muKuunganidza Tubes 2 ml.Chinja musanganiswa kubva kuChikamu chekutanga kuenda kuFastPure RNA Columns, centrifuge pa12,000 rpm (13,400 × g) kwe1 min, uye bvisa iyo filtrate.
3. Wedzera 600 μl yeBuffer RW kuFastPure RNA Columns, centrifuge pa 12,000 rpm (13,400 × g) kwe30 sec, uye bvisa filtrate.
4. Dzokorora Danho rechitatu.
5. Centrifuge mbiru isina chinhu pa12,000 rpm (13,400 × g) ye2 min.
6. Nyatsoendesa FastPure RNA Columns muRNase-isina Collection Tubes 1.5 ml (yakapihwa mukiti), uye wedzera 30 - 50 μl yeRNase-isina ddH2O pakati pemembrane pasina kubata mbiru.Bvumira kumira pakushambidzika kwemaminiti 1 uye centrifuge pa12,000 rpm (13,400 × g) kwe1 min.
7. Rasa FastPure RNA Columns.DNA / RNA inogona kushandiswa zvakananga kune assays anotevera, kana kuchengetwa pa -30 ~ -15 ° C kwenguva pfupi kana -85 ~ -65 ° C kwenguva yakareba.
Notes
Yekushandisa tsvakurudzo chete.Kwete kushandiswa mukuongorora maitiro.
1. Equilibrate samples kune kamuri tembiricha pamberi.
2. Mavhairasi anotapuriranwa zvakanyanya.Ndokumbirawo muve nechokwadi chekuti matanho ese anodiwa ekuchengetedza atorwa musati maitwa.
3. Dzivisa kudzokorora kutonhora uye kunyunguduka kwemuenzaniso, sezvo izvi zvinogona kutungamirira pakuora kana kuderedza goho rehutachiwana hwakabviswa DNA / RNA.
4. Midziyo yekuzvigadzirira inosanganisira mazano epipette asina RNase, 1.5 ml RNase-free centrifuge tubes, centrifuge, vortex mixer, uye pipettes.
5. Paunenge uchishandisa kit, pfeka bhachi rerabhoritari, magirovhosi elatex anoraswa, uye mask inoraswa uye shandisa zvinoshandiswa zvisina RNase kuti uderedze njodzi yekusvibiswa kweRNase.
6. Ita nhanho dzese pane tembiricha yemumba kunze kwekunge zvatsanangurwa.
Mechanism & Workflow
