Proteinase K (Liquid)
Zvakanakira
● Kugadzikana kwepamusoro uye basa re enzyme rinobva pane inotungamirirwa tekinoroji
● Inoshivirira munyu weGuanidine
● RNase yemahara, DNase isina uye Nickase yemahara, DNA <5 pg/mg
Tsanangudzo
Proteinase K yakagadzikana serine protease ine yakafara substrate chaiyo.Iyo inosvibisa mapuroteni akawanda munharaunda yekuzvarwa kunyange pamberi pezvidziviriro.Humbowo kubva kukristaro uye mamorekuru maitiro ezvidzidzo zvinoratidza kuti enzyme ndeye subtilisin mhuri ine inoshanda saiti catalytic triad (Asp 39-His 69-Ser 224).Nzvimbo huru ye cleavage ndiyo peptide bond iri padyo necarboxyl boka re aliphatic uye anonhuwirira amino acids ane akavharika alpha amino mapoka.Iyo inowanzoshandiswa kune yayo yakafara chaiyo.
Chimiro chemakemikari
Tsanangudzo
| Test zvinhu | Zvinotsanangurwa |
| Chitarisiko(Ruvara) | Isina ruvara kune Chiedza Brown |
| Chitarisiko (Turbidity) | Zvakajeka kune Zvishoma Zvishoma Hazy |
| Chitarisiko (Fomu) | Liquid |
| Chiitiko | ≥800U/ml |
| Protein mg protein / ml | ≥20 mg / ml |
| RNase | Hapana chaonekwa |
| DNase | Hapana chaonekwa |
| Nicase | Hapana chaonekwa |
Applications
Genetic diagnostic kit;
RNA uye DNA extraction kits;
Kubviswa kwezvinhu zvisiri zveprotein kubva kumatishu, kuderedzwa kweprotein tsvina, senge
DNA vaccine uye kugadzirira heparin;
Kugadzirira kwechromosome DNA ne pulsed electrophoresis;
Western blot;
Enzymatic glycosylated albumin reagents in vitro diagnostics
Kutumira uye Kuchengeta
KutumiraAmbient
Storage Conditions:Chengetedza pa -20 ℃(Nguva refu)/ 2-8℃(Nguva pfupi)
Zuva reyedzo rinokurudzirwa:2Makore
