Multiplex Imwe Nhanho RT-qPCR Probe Kit (UDG Plus)
Tsanangudzo
Multiplex Imwe Nhanho RT-qPCR Kit (UDG Plus) ndeye probe-based RT-qPCR yekukurumidza kuona kit yakakodzera single-plex kana yakawanda-plex quantitative PCR uchishandisa RNA setemplate (senge RNA hutachiona).Inoshandiswa kukwidziridza DNA yakasungwa kaviri kubva kune imwechete stranded RNA matemplate kubvumidza kukurumidza chaiyo-nguva quantification yeRNA zvinangwa.Mune nhanho yereverse transcriptase inosanganisa mamorekuru eDNA ane stranded (cDNA) anopindirana neRNA template.
Reaction Process
Zvikamu
| Zvikamu | 200rxns | 1,000rns | 10,000 rxns |
| RNase-yemahara ddH2O | 3*1ml | 15ml | 150ml |
| 5 * imwe nhanho musanganiswa | 800μl | 4*1ml | 40ml |
| Imwe nhanho enzyme musanganiswa | 200μl | 1ml | 10ml |
| 50* ROX referenzi Dye 1 | 80μl | 400μl | 4*1ml |
| 50* ROX referensi Dye 2 | 80μl | 400μl | 4*1ml |
a.Imwe-Nhanho Buffer inosanganisira dNTP Musanganiswa uye Mg2+.
b.Enzyme Mix inonyanya kuve nereverse transcriptase, Hot Start Taq DNA polymerase (antibody modification) uye RNase inhibitor.
c.Inoshandiswa kugadzirisa kukanganisa kwefluorescene sinal pakati pematsime akasiyana.
c.ROX: Iwe unofanirwa kusarudza iyo calibration zvinoenderana nemhando yechiridzwa chekuyedza.
Applications
Kuonekwa kwezvirwere
diagnostics uye tsvakurudzo yebundu
Kuonekwa kwechirwere chemhuka
Kuongororwa kwekutanga kwezvirwere zvenhaka
Kuonekwa kwepathogenic microorganisms muzvokudya
Kutumira uye Kuchengeta
Zvekufambisa:Ice packs
Storage Conditions:Chengetedza pa -30 ~ -15 ℃.
Shief hupenyu:1 makore
