Ultra Nuclease Assay Kit (ELISA)

Cas Nha.:9025-65-4

EC.Nha.: 3.1.30.2

Package: 96 Muedzo

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Product Detail

Tsanangudzo

Iyi Ultra Nuclease ELISA kit ijecha rine ELISA rinofanira kuitwa mune microplate fomati.Sample inogona kunge iine endonuclease inoiswa mu microtiter ndiro matsime ayo akafanoputirwa neaffinity yakacheneswa anti-endonuclease capture antibody.Mushure mekuvhara uye danho rekugeza umo zvikamu zvisingabhadharwi zvinobviswa, enzyme-conjugated, anti-endonuclease detector antibody inowedzerwa.Maitiro ekudzivirira muviri anokonzera kuumbwa kwesandwich complex ye solid phase antibody-endonuclease-enzyme yakanyorwa kuti antibody.Mushure mekupedzisira kushambidza danho, substrate solution inowedzerwa kumatsime uye inogadzirisa, zvichiita kuti ruvara ruvepo.The optical density inoyerwa photometrically uye inoenderana ne
analyte concentration iripo mumatsime.Iyo endonuclease concentration mune isingazivikanwe sampuli inogona kuverengerwa zvichienderana neinoenderana chiyero curve.

Kemikari Mamiriro

Chikamu Tsanangudzo

Huwandu hwe enzyme inoshandiswa kushandura kukosha kwekunyudza kwe △A260 ne 1.0 mukati me30min.
pa 37 ° C, pH 8.0, yakaenzana neyakagayiwa 37μg salmon urume DNA kuburikidza nekucheka mu oligonucleotides, yakatsanangurwa sechinhu chinoshanda.

Kushandisa uye Dosage

• Bvisa exogenous nucleic acid kubva kune vaccine zvigadzirwa, kuderedza njodzi yasara nucleic acid muchetura uye kunatsiridza kuchengetedzwa kwechigadzirwa.
• Deredza viscosity yemvura yekudya inokonzerwa ne nucleic acid, kupfupisa nguva yekugadzira uye kuwedzera chibereko cheprotein.
• Bvisa nucleic acid iyo yakaputira chidimbu (virusi, inclusion body, etc.), iyo inobatsira kusunungurwa uye kucheneswa kwechidimbu.
• Kurapa kwenyukireya kunogona kuvandudza kugadziriswa uye kudzorerwa kwemuenzaniso wekororamu chromatography, electrophoresis uye blotting analysis.
• Mukurapa kwemajini, nucleic acid inobviswa kuti iwane mavhairasi anonatswa adeno.