Homocysteine (HCY)
Tsanangudzo
Homocysteine (HCY) inoshandiswa kuona homocysteine muropa revanhu.Homocysteine (Hcy) is sulfur-ine amino acid inogadzirwa nemethionine metabolism.80% yeHcy inosungirirwa kumapuroteni kuburikidza ne disulfide zvisungo muropa, uye chikamu chiduku chete chemahara homocysteine chinotora chikamu mukutenderera.Hcy mazinga anowirirana zvakanyanya nezvirwere zvemoyo.chinhu chakakosha chengozi yechirwere chemoyo.Kuwedzera kweHcy muropa kunomutsa madziro emudziyo weropa kukonzeresa kukanganisa kumudziyo we arterial, zvichitungamira mukuzvimba uye kuumbwa kweplaque pamadziro emudziyo, izvo zvinozopedzisira zvaita kuti kuvharika kwekuyerera kweropa mumoyo.Mune varwere vane hyperhomocystinuria, kukanganisa kwakakomba kwemajini kunokanganisa Hcy metabolism, zvichiita kuti hyperhomocysteinemia.Kusakwana kwemajini kana kushomeka kwekudya kwevhithamini B kuchaperekedzwa nehupamhi hwepakati kana hunyoro hweHcy, izvo zvinowedzerawo njodzi yechirwere chemoyo.Yakakwira Hcy inogonawo kukonzera kuremara kwekuzvarwa senge neural tube defects uye congenital malformations.
Kemikari Mamiriro
Test musimboti
Oxidized Hcy inoshandurwa kuita yemahara Hcy, uye yemahara Hcy inopindirana ne serine pasi pecatalysis yeCBS kugadzira L-cystathionine.L-cystathionine inogadzira Hcy, pyruvate uye NH3 pasi pecatalysis yeCBL.Iyo pyruvate inogadzirwa neiyi cycle reaction inogona kuwonekwa nelactate dehydrogenase LDH neNADH, uye chiyero chekushandura cheNADH kuenda kuNAD chakanangana neiyo Hcy zvirimo mumuenzaniso.
Kutakura uye kuchengetedza
Zvekufambisa:2-8°C
Nguva yekuchengetedza uye yechokwadi:Reagents dzisina kuvhurwa dzinofanira kuchengetwa pa 2-8 ° C murima, uye nguva yechokwadi mwedzi gumi nemaviri;mushure mekuvhura, ma reagents anofanira kuchengetwa murima pa 2-8 ° C, uye nguva yekutendeseka ndeye 1 mwedzi pasi pemamiriro ekusava nekusvibiswa;iyo reagents haifanirwe kuve nechando.
Cherechedza
Sample zvinodiwa: Sample is fresh serum kana plasma (heparin anticoagulation, 0.1mg heparin inogona anticoagulate 1.0ml ropa).Ndokumbira uise centrifuge plasma nekukurumidza mushure mekuunganidzwa kweropa, kana refrigerate uye centrifuge mukati 1 awa.
