Proteinase K (Lyophiled Powder)
Zvakanakira
● Kugadzikana kwepamusoro uye basa re enzyme rinobva pane inotungamirirwa tekinoroji
● Inoshivirira munyu weGuanidine
● RNase yemahara, DNase yemahara uye Nickase yemahara,DNA <5 pg/mg
Tsanangudzo
Proteinase K yakagadzikana serine protease ine yakafara substrate chaiyo.Iyo inosvibisa mapuroteni akawanda munharaunda yekuzvarwa kunyange pamberi pezvidziviriro.Humbowo kubva kukristaro uye mamorekuru maitiro ezvidzidzo zvinoratidza kuti enzyme ndeye subtilisin mhuri ine inoshanda saiti catalytic triad (Asp 39-His 69-Ser 224).Nzvimbo huru ye cleavage ndiyo peptide bond iri padyo necarboxyl boka re aliphatic uye anonhuwirira amino acids ane akavharika alpha amino mapoka.Iyo inowanzoshandiswa kune yayo yakafara chaiyo.
Chimiro chemakemikari
Tsanangudzo
Test zvinhu | Zvinotsanangurwa |
Tsanangudzo | Ichena kusvika kuchena amorphous powder, Lyophilied |
Chiitiko | ≥30U/mg |
Solubility(50mg Powder/mL) | Pachena |
RNase | Hapana chaonekwa |
DNase | Hapana chaonekwa |
Nickase | Hapana chaonekwa |
Applications
Genetic diagnostic kit;
RNA uye DNA extraction kits;
Kubviswa kwezvinhu zvisiri zveprotein kubva kumatishu, kuderedzwa kweprotein tsvina, senge
DNA vaccine uye kugadzirira heparin;
Kugadzirira kwechromosome DNA ne pulsed electrophoresis;
Western blot;
Enzymatic glycosylated albumin reagents in vitro diagnostics
Kutumira uye Kuchengeta
KutumiraAmbient
Storage Conditions:Chengetedza pa -20 ℃(Nguva refu)/ 2-8℃(Nguva pfupi)
Zuva reyedzo rinokurudzirwa:2Makore
Zvekungwaririra
Pfeka magirovhosi uye magirazi ekudzivirira paunenge uchishandisa kana kuyera, uye gara uine mhepo yakanaka mushure mekushandisa.Ichi chigadzirwa chinogona kukonzera allergic reaction.Kukonzera kushatirwa kwemaziso kwakakomba.Kana inhaled, inogona kukonzera allergies kana asthma zviratidzo kana dyspnea.Inogona kukonzera kushatirwa kwekufema.
Assay Unit Tsanangudzo
Imwe unit (U) inotsanangurwa sehuwandu hwe enzyme inodiwa ku hydrolyze casein kugadzira 1 μmol tyrosine paminiti pasi pemamiriro anotevera.