Multiplex PCR Taq DNA Polymerase

Multiplex PCR DNA Polymerase chizvarwa chitsva chekupisa-kutanga DNA polymerase yakavakirwa pakugadzirisa antibody uye yakakwidziridzwa kuvandudza template kuwirirana.Iyo Yakakwira senitivity vhezheni yakanyatso gadziridzwa kuti multiplex PCR ine simba rekusimudzira kugona uye yakakwirira yekuona senitivity.Iine yakakwana buffer system yemultiplex PCR, inoenderana nehuwandu hwakawanda hwechigadzirwa GC zvemukati uye primer Tm kukosha.Iyo yakawanda yakawanda yemultiplex PCR assays inogona kuitwa patembiricha yepasirese ye60°C pasina imwe optimization.

Ichi chigadzirwa chine kukwirira kwakanyanya kwekusimudzira, uye panguva imwe chete inogona kukwidziridza zvidimbu zvakanangwa muhuwandu hwe50 - 3,500 bp;ine simba rekusimudzira simba, uye inogona kuita hupfumi hwemazana akati wandei kana anopfuura amplifications;kushivirira kwakanakisa kwekusachena, kunopesana zvakanyanya nemhando dzakasiyana-siyana dze inhibitors uye kusachena, uye inopindirana nekuwedzerwa kwakananga kweropa rose, makadhi eropa, nezvimwewo. Mareagents ane kugadzikana kwakanaka, kushandiswa kwakakura, uye anowirirana nemhando dzakasiyana-siyana dzekuona.