Rnase Inhibitor (Glycerol isina)
Murine RNase inhibitor is recombinant murine RNase inhibitor inoratidzwa nekucheneswa kubva kuE.coli.Inosungira kuRNase A, B kana C muchiyero che1: 1 kuburikidza nekubatana kusina-covalent, nokudaro kuvharidzira basa rema enzymes matatu uye kudzivirira RNA kubva pakuora.Nekudaro, Izvo hazvishande kurwisa RNase 1, RNase T1, S1 Nuclease, RNase H kana RNase kubva kuAspergillus.Murine RNase inhibitor yakaedzwa neRT-PCR, RT-qPCR uye IVT mRNA, uye yaienderana neakasiyana ekutengesa Reverse transcriptases, DNA polymerases uye RNA polymerases.
Inofananidzwa nevanhu RNase inhibitors, murine RNase inhibitor haina ma cysteine anonyanya kunetseka kune oxidation iyo inokonzera kusagadziriswa kweiyo inhibitor.Izvo zvichiita kuti zvigadzikane pakadzika kudzika kweDTT (pasi pe1 mM).Iyi ficha inoita kuti ive yakakodzera kushandiswa mukuita uko yakanyanya kuwanda kweDTT inopesana nemaitiro (semuenzaniso Real-time RT-PCR).
Application
Ichi chigadzirwa chinogona kushandiswa zvakanyanya mune chero kuyedza uko RNase kupindira kunogoneka kudzivirira kusvibiswa kweRNA, senge:
1.First-strand cDNA synthesis, RT-PCR, RT-qPCR, nezvimwewo;
2.Chengetedza RNA kubva pakuora mu in-vitro transcription/shanduro (eg viral replication in vitro);
3.Kuvharidzirwa kwebasa reRNase panguva yekuzviparadzanisa neRNA nekucheneswa.
Storage Conditions
Chengetedza pa -25～-15℃;
Freeze-thaw cycles ≤ 5 nguva;
Inoshanda kwemakore 1.
Tsanangudzo yeyuniti
Chimwe chikamu chinotsanangurwa sehuwandu hweRNase Inhibitor hunodiwa kuti inhibite basa re5 ng yeRNase A ne50%.
Molecular uremu
RNase Inhibitor (Glycerol-isina) ipuroteni ye50 kDa.
Kudzora kwehutano
Exonuclease Chiitiko:
Kuiswa kwe 40 U ye enzyme ine 1 μg λ-Hind III digest DNA kwemaawa gumi nematanhatu pa 37 ° C yakakonzera kusaonekwa kwekuora kweDNA sekutsanangurwa negel electrophoresis.
Endonuclease Chiitiko:
Kuiswa kwe 40 U ye enzyme ine 1 μg λ DNA kwemaawa gumi nematanhatu pa 37 ° C kwakaguma nekusaona kusvibiswa kweDNA sekutsanangurwa negel electrophoresis.
Nicking Chiitiko:
Kuiswa kwe 40 U ye enzyme ine 1 μg pBR322 kwemaawa gumi nematanhatu pa37 ℃ kwakakonzera kusaonekwa kusvibiswa kweDNA sekutsanangurwa negel electrophoresis.
RNase Chiitiko:
Kuiswa kwe 40 U ye enzyme ine 1.6 μg MS2 RNA kwemaawa mana pa37 ℃ kwakakonzera kusaonekwa kwekuora kweRNA sekutsanangurwa negel electrophoresis.
E.coli DNA:
40 U yeEnzyme inowonekwa naTaqMan qPCR.Iyo E.coli DNA ndeye ≤ 0. 1pg/40U.
Notes
1.Usazunungusa kana kubvongodza nechisimba kudzivirira kusashanda kwe enzyme.
2.RNase inhibitor inoshanda pane tembiricha kubva ku25 ℃ kusvika ku55 ℃ uye haina inactivated pa ≥65 ℃.
3.Kuitwa kweRNase H, RNase 1, uye RNase T1 hakuna kudziviswa.
4.The pH range yekudzivisa RNase basa rakawanda (inoshanda pa pH 5-9), ichiratidza chiitiko chepamusoro pa pH 7-8.
