Sample Release Reagent
Sample Release Reagent ndeye molecular POCT diagnostic scenarios.Kune iwo maviri masisitimu eakananga amplification LAMP uye yakananga amplification PCR, hapana chikonzero chekubvisa nucleic acid.Iyo crude lysate yemuenzaniso inogona kukwidziridzwa zvakananga, iyo inotarirwa gene inogona kunyatsoonekwa, iyo sampuli yekuona nguva inogona kupfupikiswa, iyo inonyatsoenderana nezvinodiwa zvekushandisa zvemolecular POCT.Inokodzera mhuno swabs, huro swabs uye mamwe mhando mhando.Iwo masampula akagadziridzwa anogona kushandiswa zvakananga kune chaiyo-nguva fluorescence kuwanda PCR kana LAMP yekuona, uye mibairo yakafanana seyakajairwa nzira yekubvisa inogona kuwanikwa pasina kuomesesa nucleic acid yekubvisa mashandiro.
Storage Conditions
Kutakura uye chengetedza pane tembiricha yekamuri.
Kudzora kwehutano
Kushanda kuona - kuwanda qPCR: Iyo 800μl Sample Release Reagent system yakakwidziridzwa.
ine 1000 makopi Novel Pseudovirus, imwe nasal swab sampu, zvichikonzera yakafanana amplification curves uyeΔCt maitiro mukati me ± 0.5 Ct.
Experimental Procedures
1. Tora 800 μl Sample Release Reagent uye paradzira mhinduro ye lysis mu 1.5 mL sampling chubhu.
2. Tora swab yemhuno kana kuti pahuro neshavi;Nzira yekuongorora nasal swab: tora swab isina utachiona woiisa mumhino, zvishoma nezvishoma famba kusvika pakadzika 1.5 cm, zvinyoro tenderera ka4 uchitarisana nenasal mucosa kweanopfuura masekonzi gumi nemashanu. , wobva wadzokorora kushanda kwakafanana pane imwe nasal mhango pamwe chete swab.Throat swab sampling maitiro: tora sterile swab uye zvinyoronyoro, nokukurumidza kupukuta tonsils pharyngeal uye shure pharyngeal rusvingo 3 nguva.
3.Isa swab nekukasika muchubhu yekuenzanisira.Musoro we swab unofanirwa kutenderedzwa uye kusanganiswa mune yekuchengetera mhinduro kweinenge 30 masekonzi kuti ive nechokwadi chekuti sampuli yakanyatsoburitswa muchubhu yesampling.
4. Incubation pane tembiricha yekamuri (20 ~ 25 ℃) ye1min, kugadzirira kwe lysis buffer kunopedzwa.
5. Zvose 25μl system RT-PCR uye RT-LAMP yaienderana ne10μl yakawanda ye template yekuwedzera yekuongorora kuedza.
Notes
1. Nhamba shoma yemuenzaniso yakananga lysate inoenderana nekamwe swab inogona kugadziriswa ku400μl, iyo inogona kugadziriswa maererano nezvinodiwa zvekuedza.
2. Kamwe sampuli yakagadziriswa nemuenzaniso wekusunungurwa reagent, inokurudzirwa kuti iite chikamu chinotevera chekuedzwa nokukurumidza, nguva yekumirira nguva inenge isingasviki 1 awa.
3. Iyo pH yemuenzaniso lysate ine acidic, uye iyo yekuona system inoda kuve neimwe buffer.Iyo yakakodzera kune yakawanda PCR, RT-PCR, uye LAMP fluorescence kutariswa ine pH buffer, asi haina kukodzera LAMP colorimetric yekuona isina buffer.
