RT-LAMP Fluorescent (Lyophilized bhora)
RT-LAMP Fluorescent Master Mix (Lyophilized Powder) ine reaction buffer, Bst DNA polymerase uye heat-resistant reverse transcriptase, lyophilized protectant, uye fluorescent dhayi.Chigadzirwa chacho chiri muchimiro che lyophilized beads, uye chete primers uye templates inoda kuwedzerwa kuti ishandiswe.
Reagent Chikamu
RT-LAMP Fluorescent Master Mix (Lyophilized Powder)
Usage
YeDNA kana RNA isothermal amplification
Storage Conditions
Kutakura uye chengetedza pa2~8°C.
Sherufu upenyu: 12 mwedzi.
Protocol
1. Bvisa nhamba inoenderana neiyo lyophilized bead zvichienderana nehuwandu hwebvunzo.
2. Rection system kugadzirira
| Chikamu | Dosage |
| RT-LAMP Fluorescent Master Mix (Lyophilized Powder) | 2 bead |
| Fluorescent dhayi | x μL |
| 10 × Primer Mix a | 5 μL |
| Matemplate DNA/ RNA b | n μL |
| Total | 50 μL |
Notes:
1) a.10 × Primer Mix : 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Loop F / B;
2) b.Iyo nucleic acid template inokurudzirwa kunyungudika mumvura yeDEPC.
Hyasen Biotechnology Co., Ltd
3) Rection purogiramu
| Tembiricha | Nguva | Cycles |
| 65℃ | 60s a | 30 |
Notes:
1. Unganidza chiratidzo chefluorescent
Notes
1. Tembiricha yekupindura inogona kugadziridzwa pakati pe62 ° C uye 68 ° C zvichienderana neprimer.mamiriro;
2. Kuedza kunofanirwa kuenzanisa kushanda, kusanganisira kugadzirira kwemaitiro system,sampuli kugadzirisa uye dosing;
3. Kuti urege kusvibiswa, zvinokurudzirwa kugadzirira maitiro ekugadzirisa mune imwe tafura ye-ultra-cleanuye wedzera matemplate kune fume hood mune mamwe makamuri kudzivirira kupindira kwenhema kwakanaka.
