RT-LAMP Colormetric Master Mix HCB5204A
Ichi chigadzirwa chine reaction buffer, RT-Enzymes Mix (Bst DNA polymerase uye heat-resistant reverse transcriptase), lyophilized Protectants uye chromogenic dhayi zvikamu.Kushandisa, ingoshandisa Buffer, iyo reaction enzyme uye primer inosanganiswa uye yakawedzerwa kune template;kuwedzera lyophilized inodzivirira inogona kuve yakatwasuka.Yakanga yakabatana ne lyophilizer uye lyophilized, uye chete primers uye templates yakawedzerwa kana yakashandiswa.Iyi kit inopa kukurumidza, kwakajeka kwekuona kuonekwa kwekusimudzira, iyo yakaipa inoratidzira inoratidzwa mutsvuku uye maitiro akanaka inoratidzwa nekuchinja kune yellow.
Chikamu
| Chikamu | HCB5204A-01 | HCB5204A-02 | HCB5204A-03 |
| Loop-mediated Amplification Buffer (ine dhayi) | 0.96 mL | 4.80 mL×2 | 9.60 mL×10 |
| RT-Enzymes Mix | 270 μL | 2.70 mL | 2.70 mL×10 |
| Lyophilized protectant | 0.96 mL×2 | 9.60 mL×2 | 9.60 mL×20 |
Applications
YeDNA kana RNA isothermal amplification.
Storage Conditions
Inotakurwa neDry ice, yakachengetwa pa -25 ~ -15 ℃.Dzivisa kazhinji kutonhora-nyoro, chigadzirwa chinoshanda kwemwedzi gumi nemaviri.
Protocol
1.Nyora iyo reaction buffer kuti ishandiswe pakamuri tembiricha.Vortex muchidimbu kana invert machubhu kakawanda kuti asanganise zvakakwana, wozoti centrifuge kuunganidza mvura kusvika kuzasi kwechubhu.
2.Kugadzirira kwekuita maitiro.Iyi reagent inogona kugadzirwa mune maviri maitiro masisitimu, liquid reaction musanganiswa uye lyophilized system musanganiswa.
1) Gadzirira mvura yekuita musanganiswa
| Chikamu | Volume |
| Loop-mediated Amplification Buffer (ine dhayi) | 10 μL |
| RT-Enzymes Mix | 2.8 μL |
| 10 × Primer Mixa | 5 μL |
| Matemplate DNA / RNA b | × μL |
| Mvura isina Nuclease | Kusvika ku50 μL |
2) Lyophilization system mix
① Gadzirira musanganiswa we lyophilized
| Chikamu | Volume |
| Loop-mediated Amplification Buffer (ine dhayi) | 10 μL |
| Lyophilized protectant | 20 μL |
| RT-Enzymes Mix | 2.8 μL |
| Mvura isina Nuclease | Kusvika ku50 μL |
② Lyophilization: Musanganiswa wakagadzirirwa wakaitwa lyophilized mu50μL system
③ Gadzirira musanganiswa wekuita
| Chikamu | Volume |
| Lyophilized mix | 1 chidimbu |
| 10 × Primer Mixa | 5 μL |
| Matemplate DNA / RNA b | × μL |
| Mvura isina Nuclease | Kusvika ku50 μL |
Notes:
1) a.10 × Primer Mix : 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Loop F / B;
2) b.DEPC (mvura yakanyungudika) inokurudzirwa kune nucleic acid templ.
1.Isa pa 65 ° C kwe 30-45mins, iyo inogona kuwedzerwa zvakakodzera maererano nekuchinja kwemavara Reaction time.
2.Sekuona ziso, yero yaive yakanaka uye tsvuku yaive negative.
Notes
1.Munyu unogona kuoneka kuzasi kwebuffer chubhu, vortex muchidimbu kana kutenderedza machubhu kakawanda kuti unyatso kusanganiswa patembiricha yepakamuri.
2.Iyo yekudziya tembiricha inogona kugadziridzwa pakati pe62 ℃ uye 68 ℃ zvinoenderana nemamiriro ekutanga.
3.Izvo zvakaputirwa reagents hazvifanirwe kuratidzwa kumhepo kwenguva yakareba.
4.Iyo tsvuku uye yero discoloration reaction inoenderana ne pH shanduko yemaitiro system, ndapota usashandise ine Tris nucleic acid yekuchengetedza mhinduro, inokurudzirwa kushandisa ddH.2O yakachengetwa nucleic acid;
5.Kuedza kunofanirwa kuve kwakamisikidzwa, kusanganisira kugadzirira kwemaitiro system, lyophilization, uye sampuli yekugadzirisa uye sampuli yekuwedzera maitiro;
6.Kuti udzivise kusvibiswa, zvinokurudzirwa kugadzirira maitiro ekugadzirisa mune imwe bhenji yakachena-yakachena, mune imwe Wedzera ma templates kune fume hood yekamuri kuti urege kupindira kwenhema.
