Ribonuclease A (RNase A)

Ribonuclease A (RNase A) i polypeptide ine tambo imwe chete ine 4 disulfide bond ine molecular huremu hunosvika 13.7 kDa.RNase A inonzi endoribonuclease iyo inonyanya kushatisa imwe-stranded RNA paC uye U zvakasara.Kunyanya, iyo cleavage inoona phosphodiester bond inoumbwa ne5'-ribose ye nucleotide uye phosphate boka pa 3'-ribose yepedyo pyrimidine nucleotide, kuitira kuti 2', 3' - Cyclic phosphates inogadziriswa nehydrolyzed kune inoenderana. 3'-nucleoside phosphates (semuenzaniso, pG-pG-pC-pA-pG inotsemurwa neRNase A kugadzira pG-pG-pCp uye A-PG).RNase A ndiyo inonyanya kushanda mukutsemura imwe-stranded RNA uye inoshanda mumamiriro ezvinhu akasiyana-siyana: pakudzika kwemunyu wakaderera (0 kusvika 100 mM NaCl), inogona kushandiswa kutsemura RNA ine tambo imwe chete, RNA ine tambo mbiri, uye RNA tambo muRNA-DNA mahybrids.nepo iine munyu wakanyanya (≥0.3 M), RNase A inogona kunyatso patsanura imwe-stranded RNA.