Imwe Nhanho RT-qPCR probe kit
Tsanangudzo
U+ Imwe Nhanho RT-qPCR Probe Kit (Glycerol-isina) is a glycerol-isina-danho rimwe RT-qPCR reagent inoshandisa RNA setemplate (yakadai seRNA virus), iyo inokodzera kusimudzira nekugadzira zvigadzirwa zve lyophilized.Ichi chigadzirwa chinosanganisa kuita kwepamusoro kwenhanho imwe yakatsaurirwa Reverse Transcriptase uye inopisa-kutanga Champagne Taq DNA Polymerase, ine yakanyatso kuomeswa-kuomesa kemikari buffer, iyo ine zvirinani amplification kunyatsoita, chiyero uye chaiyo, uye inoenderana nekuomesa-kuomesa nechando. maitiro.Kuwedzera, iyo dUTP / UDG inopesana nekusvibiswa system inopinzwa mu reagent, iyo inogona kushanda pakamuri tembiricha, kubvisa kukanganiswa kweamplification chigadzirwa kusvibiswa paqPCR, uye kuve nechokwadi chechokwadi chemhedzisiro.
Nheyo dzekutanga dzeRT-qPCR
Tsanangudzo
| Test Items | Tsanangudzo | Mhedzisiro |
| (SDS PEJI) Kuchena kweEnzyme Stock (SDS PEJI) | ≥95% | Pass |
| Endonuclease Chiitiko | Haisati yaonekwa | Pass |
| Exodulease Chiitiko | Haisati yaonekwa | Pass |
| Rnase Chiitiko | Haisati yaonekwa | Pass |
| Residual E.coli DNA | ＜1makopi/60 | Pass |
| Functional Assay-System | 90%≤110% | Pass |
Zvikamu
| Zvikamu | 100rxns | 1,000rns | 5,000 rxns |
| RNase-yemahara ddH2O | 2*1ml | 20ml | 100ml |
| 5 * imwe nhanho musanganiswa | 600μl | 6*1ml | 30ml |
| Imwe nhanho enzyme musanganiswa | 150μl | 2*750μl | 7.5ml |
| 50* ROX referenzi Dye 1 | 60μl | 600μl | 3*1ml |
| 50* ROX referensi Dye 2 | 60μl | 600μl | 3*1ml |
a.Imwe-Nhanho Buffer inosanganisira dNTP Musanganiswa uye Mg2+.
b.Enzyme Mix inonyanya kuve nereverse
transcriptase, Kupisa Kutanga Taq DNA polymerase (antibody modification) uye RNase inhibitor.
c.Inoshandiswa kugadzirisa kukanganisa kwefluorescene sinal pakati pematsime akasiyana.
c.ROX: Iwe unofanirwa kusarudza iyo calibration zvinoenderana nemhando yechiridzwa chekuyedza.
Applications
Kuonekwa kweQPCR
Kutumira uye Kuchengeta
Zvekufambisa:Ice packs
Storage Conditions:Chengetedza pa -20 ℃.
Shief Hupenyu:18months
