Phosphatase Alkaline (ALP)
Tsanangudzo
Alkaline Phosphatase inotorwa kubva kune recombinant E. coli strain inotakura jena reTAB5.Iyo enzyme inokonzera dephosphorylation ye5' uye 3' magumo eDNA uye RNA phosphomonoesters.Zvakare, iyo hydrolyses ribose, pamwe ne deoxyribonucleoside triphosphates (NTPs uye dNTPs).TAB5 Alkaline Phosphatase inoshanda pa 5' yakasimudzwa, 5' yakadzokororwa uye yakagomara magumo.Iyo Phosphatase inogona kushandiswa mune akawanda mamorekuru biology application, senge cloning kana probe end labeling kubvisa phosphorylated magumo eDNA kana RNA.Mukuedza kwecloning, dephosphorylation inodzivirira iyo linearized plasmid DNA kubva pakuzvibatanidza.Inogona zvakare kudzikisa zvisina kubatanidzwa dNTPs muPCR maitiro kugadzirira template yeDNA kutevedzana.Iyo enzyme haigadziriswe zvachose uye isingachinjiki nekudziya pa70 ° C kwemaminetsi mashanu, zvichibva zvaita kuti kubviswa kwephosphatase kusati kwasungwa kana kupedzisa kunyora zvisina kufanira.
Usage
1.Alkaline phosphatase yakabatana nemapuroteni (antibodies, streptavidin etc,) inogona kunyatsoona chinangwa che molecules, uye inogona kushandiswa muELISA, WB uye histochemical discovery;
2.Alkaline phosphatase inogona kushandiswa dephosphorize iyo 5 '-terminal yeDNA kana RNA kudzivirira kuzvibatanidza;
3.Iyo pamusoro apa dephosphorylated DNA kana RNA inogona kunyorwa neredhiyo-yakanyorwa phosphates (kuburikidza neT4 poly-nucleotide kinase)
Mushonga Wemakemikari
Tsanangudzo
| Test Items | Zvinotsanangurwa |
| Enzyme Chiitiko | 5U/μL |
| Endonuclease Chiitiko | Haisati yaonekwa |
| Exonuclease Chiitiko | Haisati yaonekwa |
| Nicking Chiitiko | Haisati yaonekwa |
| RNase Chiitiko | Haisati yaonekwa |
| E.coli DNA | ≤1kopi/5U |
| Endotoxin | LAL-Muedzo, ≤10EU/mg |
| Kuchena | ≥95% |
Kutakura uye kuchengetedza
Transportation:AmbIent
Storage :Chengetedza pa2-8°C
Inokurudzirwa kuongorora zvakareHupenyu:2 gore
