Peroxidase (Horseradish source) Synonym: Hydrogen peroxide oxidoreductase;HRP

Horseradish peroxidase (HRP) yakaparadzaniswa kubva pamidzi yehorseradish (Amoracia rusticana) uye ndeyeboka referroprotoporphyrin re peroxidases.HRP inosanganiswa nyore nyore nehydrogen peroxide (H2O2).Iyo inoguma [HRP-H2O2] yakaoma inogona oxidize akasiyana siyana evanopa mahydrogen:
Donor + H2O2 → Oxidized Donor + 2 H2O
HRP ichaita oxidize akasiyana substrates (ona Tafura 1):
• Chromogenic
• Chemiluminescent (yakadai se luminol kana isoluminol)
• Fluorogenic (senge tyramine, homovanillic acid, kana 4-hydroxyphenyl acetic acid)
HRP iketani imwe chete polypeptide ine mabhiriji mana disulfide.HRP inonzi glycoprotein ine 18% carbohydrate.Kuumbwa kwecarbohydrates kunosanganisira galactose, arabinose, xylose, fucose, mannose, mannosamine, uye galactosamine, zvichienderana neisozyme chaiyo.

HRP ishizha rinoshandiswa zvakanyanya kune immunoglobulins mune akawanda akasiyana immunochemistry application, kusanganisira immunoblotting, immunohistochemistry, uye ELISA.HRP inogona kubatanidzwa kune masoja ekudzivirira chirwere nenzira dzakasiyana siyana, kusanganisira glutaraldehyde, periodate oxidation, kuburikidza ne disulfide bond, uye zvakare kuburikidza neamino uye thiol inonangirwa kuyambuka-linkers.HRP ndiyo inonyanya kudiwa label yemasoja ekudzivirira chirwere, sezvo iri diki uye yakatsiga pane matatu ane mukurumbira maenzayemu labels (peroxidase, β-galactosidase, alkaline phosphatase) uye glycosylation yayo inotungamira kuderera kusinganyatso sunga.Ongororo ye glutaraldehyde uye periodate conjugation nzira dzakaburitswa.

Peroxidase inoshandiswawo kugadzirisa glucose4 uye peroxides mumhinduro.Zvinyorwa zvakati wandei, 6-24 thes, 25-29 uye dissertations 30-46 vakataura kushandiswa kweP8375 mumapuroteni avo ekutsvagisa.