Glycohemoglobin A1c ( HbA1c) Test Kit
Zvakanakira
● Kururama kwepamusoro
● Simba rekurwisa-kupindira kwakasimba
● Kugadzikana kwakanaka
Kemikari Mamiriro
Applications
In vitro bvunzo yehuwandu hwekutsunga kweHbA1c kusungwa muropa revanhu rese pane photometric system.HbA1c chigadzirwa che hemoglobin (Hb) inogadzira inononoka uye inoenderera isiri-enzymatic glycation reaction pasi pehuwandu hweglucose yeropa.Glucose inoshandura hemoglobin kunyanya mune yayo n-terminal valine residu kuti iumbe glycated hemoglobin.Pasi pezvakajairwa mamiriro emuviri, kugadzirwa kweasiri-enzymatic glycosylation reaction zvigadzirwa zvakanaka kuenzana nekusangana kwezvinoita.Sezvo hemoglobin concentration inoramba yakagadzikana, glycosylation mazinga anonyanya kuenderana neglucose concentration uye zvakare ane hukama nehurefu hwehemoglobin uye glucose kuratidzwa.Naizvozvo, HbA1c chiratidzo chakanaka kuavhareji yeglucose yeropa yevarwere kwemwedzi miviri kusvika mitatu yekupedzisira.
Principle
Pasi pechiito cheprotease, n-terminal ye β chain muHbA1c inogurwa uye glycosylated dipeptides inoburitswa.Mukuita kwekutanga, Hb concentration inogona kuwanikwa nekuyera kunyura kwe480 nm.Muchiitiko chechipiri, fructosyl peptide oxidase (FPOX) inoshanda pa glycosylated dipeptides kugadzira hydrogen peroxide iyo inogona kuita nechromogenic agents kuti ibudise kunyura pa 660nm pamberi peroxidase, ipapo kusangana kweHbA1c kunogona kuwanikwa nekuyera kunyura kwe. 660nm.Zvinoenderana neiyo yakawanikwa HbA1c yekusangana uye Hb concentration, iyo percentage yeHbA1c (HbA1c%) inogona kuverengerwa.
Inoshanda
Hitachi 7180/7170/7060/7600 otomatiki biochemical analyser、Abbot 16000、OLYMPUS AU640automatic biochemical analyzer
Reagents
| Zvikamu | Concentrations |
| Reagent 1(R1) | |
| Zvakanaka buffer | 100mmol/L |
| PRK | 500KU/L |
| DA-67 | 10mmol/L |
| Reagents 2 (R2) | |
| Zvakanaka buffer | 100mmol/L |
| Fructosyl peptide oxidase | 50 KU/L |
| Reagent 3(R3) | |
| Zvakanaka buffer | 100mmol/L |
Kutakura uye kuchengetedza
Zvekufambisa:Ambient
Kuchengetedza uye Kugadzikana:
Kusvika pakupera kwemazuva anoratidzwa pane iyo label, kana yakachengetwa isina kuvhurwa pa2-8 ℃ uye yakachengetedzwa kubva kuchiedza.Kana yangovhurwa, ma reagents anogadzikana kwemazuva makumi maviri nemasere kana akaiswa mufiriji pane analyzer kana firiji.
Kusvibiswa kwema reagents kunofanira kudziviswa.Usaomesa reagents.
Kana yanyungudutswa, iyo calibrator yakagadzikana kwemazuva gumi nemashanu pa2-8 ℃, kutonga kunogadzikana kwemazuva manomwe pa2-8 ℃, usaomesa.
Sherufu Hupenyu:1 gore
