Imwe Nhanho RT-qPCR probe kit
Tsanangudzo
Imwe Danho qRT-PCR Probe Kit yakanyatsogadzirirwa qPCR inoshandisa zvakananga RNA (eg virus RNA) setemplate.Uchishandisa gene specific primers (GSP), iyo reverse transcription uye qPCR inogona kupedzwa muchubhu imwe, ichideredza zvakanyanya maipiping maitiro uye njodzi yekusvibiswa.Inogona kuvharwa pa55 ℃ isingakanganise kushanda uye kunzwa kweqRT-PCR.Kubatanidza kuita kwepamusoro kweHiScript III Reverse Transcriptase uye inopisa-kutanga Champagne Taq DNA Polymerase, ine yakagadziridzwa buffering system, kunzwisiswa kweOne Step qRT-PCR Probe Kit kunogona kusvika 0.1 pg yeRNA yakazara kana asingasviki gumi makopi eRNA matemplate. uye ine kugadzikana kwepamusoro kwekushisa.Imwe Nhanho qRT-PCR Probe Kit inopihwa muMaster Mix.Iyo 5 × One Danho Musanganiswa ine optimized buffer uye dNTP/dUTP Musanganiswa, uye yakakodzera kune yakakwirira-specificity yekuona masisitimu zvichienderana nefluorescence yakanyorwa probes (eg TaqMan).
Reaction Process
Zvikamu
| Zvikamu | 100rxns | 1,000rns | 5,000 rxns |
| RNase-yemahara ddH2O | 2*1ml | 20ml | 100ml |
| 5 * imwe nhanho musanganiswa | 600μl | 6*1ml | 30ml |
| Imwe nhanho enzyme musanganiswa | 150μl | 2*750μl | 7.5ml |
| 50* ROX referenzi Dye 1 | 60μl | 600μl | 3*1ml |
| 50* ROX referensi Dye 2 | 60μl | 600μl | 3*1ml |
a.Imwe-Nhanho Buffer inosanganisira dNTP Musanganiswa uye Mg2+.
b.Enzyme Mix inonyanya kuve nereverse
transcriptase, Kupisa Kutanga Taq DNA polymerase (antibody modification) uye RNase inhibitor.
c.Inoshandiswa kugadzirisa kukanganisa kwefluorescene sinal pakati pematsime akasiyana.
c.ROX: Iwe unofanirwa kusarudza iyo calibration zvinoenderana nemhando yechiridzwa chekuyedza.
Applications
Kuonekwa kwezvirwere
diagnostics uye tsvakurudzo yebundu
Kuonekwa kwechirwere chemhuka
Kuongororwa kwekutanga kwezvirwere zvenhaka
Kuonekwa kwepathogenic microorganisms muzvokudya
Kutumira uye Kuchengeta
Zvekufambisa:Ice packs
Storage Conditions:Chengetedza pa -30 ~ -15 ℃.
Shief hupenyu:1 makore
