Imwe Nhanho RT-qPCR SYBR Green Kit
Tsanangudzo
Universal Blue qPCR Master Mix (Dye Yakavakirwa) ndeye pre-mhinduro ye2 × chaiyo-nguva yakawanda PCR amplification inoratidzwa nehupamhi hwekunzwa uye hunhu, ibhuruu muruvara, uye ine mhedzisiro yemuenzaniso wekuwedzera kutsvaga.Iyo yakakosha chikamu Taq DNA polymerase inoshandisa antibody inopisa kutanga kuvharira kusiri-chaiyo amplification nekuda kweprimer annealing panguva yekugadzirira sampuli.Panguva imwecheteyo, fomula rinowedzera zvinhu zvinosimudzira kusimudzira kugona kwePCR kuita uye kuenzanisa kukwidziridzwa kwemajini ane akasiyana GC zviri mukati (30 ~ 70%), kuitira kuti huwandu hwePCR huwane hukama hwakanaka hwemutsara muhuwandu hwakawanda. dunhu.Ichi chigadzirwa chine yakakosha ROX Passive Reference Dye, inoshanda kune ese qPCR zviridzwa.Izvo hazvifanirwe kugadzirisa kusungirirwa kweROX pane zvakasiyana zviridzwa.Izvo zvinongodiwa kuwedzera maprimers uye matemplate kugadzirira maitiro sisitimu yekusimudzira.
Reaction Process
Tsanangudzo
| Itens | 50T |
| SYBR Green buffer | 500 μL |
| Enzymes | 50 μL |
| 50 × High Rox | 50 μL |
| 50×Low Rox | 50 μL |
| RNase-yemahara ddH2O | 500 μL |
Applications
Gene Expression
Kutumira uye Kuchengeta
Zvekufambisa:Ice packs
Storage Conditions:Kuchengetedza pa -20°℃
Zuva reyedzo rinokurudzirwa:18 mwedzi
