Hotstart Taq DNA Polymerase (5u/ul)
Taq DNA Polymerase inopisa kutanga DNA polymerase ine kuvharika kaviri nemasoja anodzivirira maviri.Ichi chigadzirwa hachingovharidzi 5′→3′ polymerase basa reTaq DNA polymerase,asi zvakare inovharira 5′→3′exonuclease chiitiko.Kupisa kwemasekonzi makumi matatu pane pre-denaturation tembiricha inogona kumisa zvachose antibody uye kuburitsa DNA polymerase chiitiko uye exonuclease chiitiko.Iyo yakapetwa kaviri inovharira hunhu haingo nyatso kudzivirira iyo nonspecific amplification inokonzerwa ne mismatch kana primer dimer, asi zvakare zvinobudirira kudzivirira kudzikira kweiyo fluorescence siginecha inokonzerwa nekudzikira kweprobe, kuitira kuti in vitro yekuona reagent igadzikane panguva yekufambisa kana kushandiswa mukamuri. tembiricha.
Zvikamu
| Chikamu | HC1012B (250U) | HC1012B (1000U) | HC1012B (10000U) | HC1012B (25000U) |
| Taq DNA Polymerase(5 U/μL) | 50 μL | 200 μL | 2 mL | 5 mL |
Storage Condition
Chigadzirwa chacho chinotumirwa nechando chakaoma uye chinogona kuchengetwa pa -25 ° C ~ -15 ° C kwemakore maviri.
Zvinotsanangurwa
| Polymerase | Taq DNA Polymerase |
| Kuchena | ≥ 95% (SDS-PEJI) |
| Sravana Sameeralu Serial 4th Hot Start | Yakavakwa-mukati Hot Start |
| Reaction Speed | Standard |
| Exonuclease Chiitiko | 5′→3′ |
Mirayiridzo
Reaction Setup
| Zvikamu | Vhoriyamu (μL) | Final Concentration |
| 2 × Buffera | 25 | 1× |
| Primer/Probe musanganiswab | × | 0.1 μmol/L-0.5 μmol/L |
| Hotstart Taq Polymerase (5U/μL) | 1.2 | 0.12 U/μL |
| DNA Templatec | × | 0.1-100 ng |
| ddH2O | Kusvikira ku50 | - |
Notes:
1) Zvinoenderana neiyo chaiyo yekuyedza application, inodiwa kugadzirira inoenderana reaction buffer.
2) Huwandu hweDNA uye huwandu hwemaprobes kana maprimers anokurudzirwa kutariswa.Iyo yakakwana yekuisa inogona kugadziridzwa zvinoenderana neakananga mamiriro ekuyedza.
Thermal cycling protocol
| Danho | Tembiricha(°C) | Nguva | Cycles |
| Pre-denaturation | 95 ℃ | 5 mins | 1 |
| Denaturation | 95 ℃ | 15 sec | 45 |
| Kuwedzera / Kuwedzera | 60 ℃a | 30 secb |
Notes:
1) Iyo tembiricha yekuita inogadziriswa zvinoenderana neiyo Tm kukosha kweiyo yakagadzirirwa primers.
2) Yakasiyana qPCR zviridzwa zvinoda akasiyana fluorescence siginecha nguva yekutora, ndapota isa zvinoenderana neiyo ipfupi nguva yakatarwa.
Notes
Ndokumbira upfeke PPE inodiwa, jasi rakadaro uye magurovhosi, kuve nechokwadi chehutano hwako uye kuchengetedzeka!
