Glucose-6-phosphate dehydrogenase (G6PDH)
Tsanangudzo
Glucose 6 phosphate dehydrogenase (G6PD) kushaikwa inhaka yenhaka umo masero matsvuku eropa anoputsika (hemolysis) apo muviri unoratidzwa kune zvimwe zvekudya, zvinodhaka, hutachiona kana kushungurudzika.Zvinoitika kana munhu asipo kana kuti ane mazinga akaderera eenzayimu glucose-6-phosphate dehydrogenase.Iyi enzyme inobatsira masero matsvuku eropa kushanda zvakanaka.Zviratidzo panguva yehemolytic episode inogona kusanganisira weti yakasviba, kuneta, kupenya, kukurumidza kwemoyo, kupfupika kwekufema, uye yellowing yeganda (jaundice).Kushaikwa kweG6PD kunogarwa nhaka nenzira yeX-yakabatana nerecessive uye zviratidzo zvinowanzoitika muvarume (kunyanya maAfrica America uye avo vanobva kune dzimwe nzvimbo dzeAfrica, Asia, uye Mediterranean).Inokonzerwa nekuchinja kwemajini muG6PD gene.
Glucose-6-Phophate Dehydrogenase (G-6-PDH) ine zvikamu zviviri zvehuremu hwema molecular akaenzana.Kutevedzana kwemaamino acid emonoma kwakabudiswa.G-6-PDH yakashandiswa mukuongororwa kwe nicotinamide adenine dinucleotide uye tissue pyridine. nucleotides.G-6-PDH inogona kuvandudzwa kubva kune urea-denatured mhinduro.
Glucose 6-phosphate dehydrogenase ndiyo yakakosha yekudzora enzyme munhanho yekutanga yepentose phosphate nzira.G-6-P-DH oxidize glucose-6-phosphate pamberi peNADP+ kubereka 6- phosphogluconate.Polyacrylamide gel electrophoresis, kusvibisa basa, uye anti-yeast G-6-PDH antibody immunoblotting zvidzidzo zvakaratidza kuti G-6-PDH iglycoprotein.
Kemikari Mamiriro
Rection Principle
D-Glucose-6-phosphate + NAD+→D-Glucono-δ-lactone-6-phosphate + NADH+H+
Tsanangudzo
| Test Items | Zvinotsanangurwa |
| Tsanangudzo | White amorphous powder, lyophilized |
| Chiitiko | ≥150U/mg |
| Kuchena(SDS-PEJI) | ≥90% |
| Solubility (10mg hupfu/ml) | Pachena |
| NADH/NADPH oxidase | ≤0.1% |
| Glutathione reductase | ≤0.001% |
| Phosphoglucose isomerase | ≤0.001% |
| Creatine phosphokinase | ≤0.001% |
| 6-Phosphogluconate dehydrogenase | ≤0.01% |
| Myokinase | ≤0.01% |
| Hexokinase | ≤0.001% |
Kutakura uye kuchengetedza
Transportation: Ambient
Storage :Chengetedza pa2-8°C
Inokurudzirwa kuongorora zvakareHupenyu:2 gore
