D-Lactate dehydrogenase (LDH)
Tsanangudzo
Iyo enzyme inoshandiswa pakubvisa pyruvate mukugadzirisa kushanda neNADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).
Usage
1.Alkaline phosphatase yakabatana nemapuroteni (antibodies, streptavidin etc,) inogona kunyatsoona chinangwa che molecules, uye inogona kushandiswa muELISA, WB uye histochemical discovery;
2.Alkaline phosphatase inogona kushandiswa dephosphorize iyo 5 '-terminal yeDNA kana RNA kudzivirira kuzvibatanidza;
3.Iyo pamusoro apa dephosphorylated DNA kana RNA inogona kunyorwa neredhiyo-yakanyorwa phosphates (kuburikidza neT4 poly-nucleotide kinase)
Mushonga Wemakemikari
Reaction mechanism
D-Lactate + NAD+→ Pyruvate +NADH + H+
Tsanangudzo
| Test Items | Zvinotsanangurwa |
| Tsanangudzo | White amorphous powder, lyophilized |
| Chiitiko | ≥200U/mg |
| Kuchena(SDS-PEJI) | ≥90% |
| Solubility (10mg hupfu/mL) | Pachena |
| NADH/NADPH oxidase | ≤0.01% |
| Malate dehydrogenase | ≤0.005% |
| Glutamate dehydrogenase | ≤0.003% |
| Pyruvate kinase | ≤0.03% |
| Glutamate Dehydrogenase | ≤0.003% |
| Aspartate aminotransferase | ≤0.001% |
| Alamine aminotransferase | ≤0.001% |
Kutakura uye kuchengetedza
Transportation:AmbIent
Storage :Chengetedza pa -20°C(Nguva refu), 2-8°C (nguva pfupi)
Inokurudzirwa kuongorora zvakareHupenyu:2 gore
