Cholesterol oxidase (COD/CHOD)
Tsanangudzo
Cholesterol oxidase (CHOD) inokonzeresa nhanho yekutanga mucholesterol catabolism.Mamwe mabhakitiriya asiri-pathogenic, akadai seStreptomyces anokwanisa kushandisa cholesterol senge kabhoni sosi.Utachiona hwepathogenic, hwakadai seRhodococcus equi, hunoda kuti CHOD iparadze macrophage yemunhu.CHOD ine bifunctional.Cholesterol inotanga kuiswa oxidized to cholest-5-en-3-one muFAD-inoda danho.The cholest-5-en-3-one is isomerized to cholest-4-en3-one.Isomerization reaction inogona kudzokororwa zvishoma.Basa reCHOD rinoenderana nemaitiro emuviri e membrane iyo iyo substrate inosungwa.
CHOD inoshandiswa kuona serum cholesterol.Ndiyo yechipiri inonyanya kushandiswa enzyme mumashandisirwo ekuongorora mushure meglucose oxidase.CHOD inowanawo kushandiswa mu microanalysis ye steroid muzvokudya zvekudya uye mukusiyanisa 3-ketosteroids kubva ku3b-hydroxysteroids.Transgenic plant inoratidzira cholesterol oxidase iri kuongororwa mukurwisana nekotoni yekotoni weevil.Cholesterol oxidase yakashandiswa zvakare seye molecular probe kujekesa cellular membrane zvimiro.
Mushonga Wemakemikari
Rection Principle
Cholesterol + O2 →△4-Cholesten-3-imwe + H2O2
Tsanangudzo
| Test Items | Zvinotsanangurwa |
| Tsanangudzo | Yellowish amorphous powder, lyophilized |
| Chiitiko | ≥8U/mg |
| Kuchena(SDS-PEJI) | ≥90% |
| Solubility (10mg hupfu/ml) | Pachena |
| Catalase | ≤0.001% |
| Glucose oxidase | ≤0.01% |
| Cholesterol esterase | ≤0.01% |
| ATPase | ≤0.005% |
Kutakura uye kuchengetedza
Transportation:Kutumirwa pasi -15°C
Storage :Chengetedza pa -25~-15°C(Nguva refu), 2-8°C(Nguva pfupi)
Inokurudzirwa kuongorora zvakareHupenyu:1 gore
