Bst 2.0 DNA Polymerase (Glycerol yemahara, yakakwira density)
Bst DNA polymerase V2 inotorwa kubva kuBacillus stearothermophilus DNA Polymerase I, iyo ine 5′→ 3′ DNA polymerase basa uye yakasimba cheni kutsiva basa, asi hapana 5′→3′ exonuclease basa.Bst DNA Polymerase V2 yakanyatsokodzera strand-displacement, isothermal amplification LAMP (Loop mediated isothermal amplification) uye kukurumidza kutevedzana.Iyi Bst DNA polymerase V2 inokwanisa kuvharidzira DNA polymerase chiitiko pakamuri tembiricha, kuitira kuti ishandiswe uye iyo reaction system inogona kugadzirwa pakudziya kwekamuri, kudzivirira kusiri-chaiyo amplification uye kuvandudza kushanda kwekuita, uye iyi vhezheni inogona. kuva lyophilized.Mukuwedzera, inokwanisa kusunungura basa rayo pamatembiricha akakwirira, nokudaro kubvisa kudiwa kweimwe nhanho yekuita.
Zvikamu
| Chikamu | HC5007A-01 | HC5007A-02 | HC5007A-03 |
| Bst DNA polymerase V2 (Glycerol-isina) (32U/μL) | 0.05 mL | 0.25 mL | 2.5 mL |
| 10 × HC Bst V2 Buffer | 1.5 mL | 2×1.5 mL | 3 × 10 mL |
| MgSO4 (100mM) | 1.5 mL | 2×1.5 mL | 2 × 10 mL |
Applications
1.LAMP isothermal amplification
2.DNA strand single displacement reaction
3.Yakakwira GC gene sequencing
4.DNA sequencing ye nanogram level.
Storage Condition
Kutakura pasi pe 0°C uye kuchengetwa pa -25°C~-15°C.
Chikamu Tsanangudzo
Chimwe chikamu chinotsanangurwa sehuwandu hwe enzyme inobatanidza 25 nmol ye dNTP mu acid isinganyunguriki zvinhu mumaminitsi makumi matatu pa 65 ° C.
LAMP Reaction
| Zvikamu | 25 μLSystem |
| 10 × HC Bst V2 Buffer | 2.5 μL |
| MgSO4 (100mM) | 1.5 μL |
| dNTPs (10mM imwe neimwe) | 3.5 μL |
| SYTO™ 16 Girini (25×)a | 1.0 μL |
| Primer mixb | 6 μL |
| Bst DNA Polymerase V2 (Glycerol-isina) (32 U/uL) | 0.25 μL |
| Template | × μL |
| ddH₂O | Kusvika ku25 μL |
Notes:
1) a.SYTOTM 16 Green (25 ×): Maererano nezvinodiwa zvekuedza, mamwe madhayi anogona kushandiswa seanotsiva;
2) b.Primer mix: inowanikwa nekusanganisa 20 µM FIP, 20 µM BIP, 2.5 µM F3, 2.5 µM B3, 5 µM LF, 5 µM LB nemamwe mavhoriyamu.
Kuita uye Mamiriro ezvinhu
1 × HC Bst V2 Buffer, incubation tembiricha iri pakati pe60°C ne65°C.
Heat Inactivation
80°C, 20mins.
