BspQI
BspQI inogona kudzokororwa kuratidzwa muE. coli iyo inogona kuziva nzvimbo chaiyo uye inogadzirwa pasi
BspQI, an IIs restriction endonuclease restriction endonuclease, inotorwa kubva kune recombinant E. coli strain inotakura yakaumbwa uye yakagadziridzwa BspQI gen kubva kuBacillus sphaericus.Inogona kuziva mawebhusaiti chaiwo, uye kutevedzana kwekucherechedzwa uye cleavage masaiti ndeaya anotevera:
5' · · · ·GCTTC(N) · · · · · · · · · · · ·3'
3' · · · · CGAGAAG(NNNN) · · · · 5'
Product Features
1. High kuita, Fast digestion;
2. Yakaderera nyeredzi chiitiko, kuve nechokwadi chekucheka kwakaringana se "scalpel";
3. Pasina BSA uye mhuka-yakabva yakasununguka;
Methylation Sensitivity
Dndine methylation:Kwete Sensitive;
Dcm methylation:Kwete Sensitive;
CpG Methylation:Kwete Sensitive;
Kuchengetedza mamiriro
Chigadzirwa chacho chinofanira kutumirwa ≤ 0 ℃;Chengetedza pa -25 ~ - 15 ℃ mamiriro.
Storage buffer
20mM Tris-HCl, 0.1mM EDTA, 500 mM KCl, 1.0 mM dithiothreitol, 500 µg/ml Recombinant Albumin, 0. 1% Trition X- 100 uye 50% glycerol (pH 7.0 @ 25°C).
Chikamu Tsanangudzo
Imwe unit inotsanangurwa sehuwandu hwe enzyme inodiwa kugaya 1µg yeInternal control DNA muawa 1 pa50 ° C muhuwandu hwekuita vhoriyamu ye50 µL.
Quality Control
Protein Purity Assay (SDS-PEJI):Kuchena kweBspQI kwaive ≥95% yakatarwa neSDS-PAGE kuongororwa.
RNase:10U yeBspQI ine 1.6μg MS2 RNA kwemaawa mana pa50 ℃ haibudisi kushatisa sekutemerwa neagarose gel electrophoresis.
Zvisiri-Yakananga DNase Chiitiko:10U yeBspQI ine 1μg λ DNA pa50℃ kwemaawa gumi nematanhatu, zvichienzaniswa ne50℃ kweawa 1, haiburitse DNA yakawandisa sekutsanangurwa kweagarose gel electrophoresis.
Ligation uye kucheka:Mushure mekugaya 1 μg λDNA ine 10U BspQI, zvidimbu zveDNA zvinogona kubatanidzwa neT4 DNA ligase pa16ºC.Uye izvi zvimedu zvakasungwa zvinogona kuchekwa zvakare neBspQI.
E. coli DNA: E. coli 16s rDNA-specific TaqMan qPCR kuonekwa kwakaratidza kuti E.coli genome residue ≤ 0.1pg/ul.
Host protein yakasara:≤ 50 ppm
Utachiona Endotoxin: LAL-yedzo, maererano neChinese Pharmacopoeia IV 2020 edition, gel muganho bvunzo nzira, general mutemo (1143).Bacterial endotoxin content inofanira kuva ≤10 EU/mg.
Reaction system uye mamiriro
| Chikamu | Volume |
| BspQ I(10 U/μL) | 1 μL |
| DNA | 1 μg |
| 10 x BspQ I Buffer | 5 μL |
| dd H2O | Kusvika ku50 μL |
Maitiro ekuita: 50 ℃, 1~16 h.
Kupisa kusashanda: 80 ° C kwe20 min.
Iyo yakakurudzirwa maitiro system uye mamiriro anogona kupa yakaringana enzyme digestion mhedzisiro, inova yekutarisa chete, ndapota tarisa kune iyo yekuedzwa mhedzisiro yeruzivo.
Product application
Kurambidza endonuclease digestion, Rapid cloning.
Notes
1. Nhamba ye enzyme ≤ 1/10 yehuwandu hwekuita.
2. Chiitiko chenyeredzi chinogona kuitika kana glycerol concentration inopfuura 5%.
3. Cleavage chiitiko chinogona kuitika kana Substrate pazasi reshiyo yakakurudzirwa.
