Ultra Nuclease
UltraNuclease is a genetically engineeredendonuclaese yakabva kuSerratia marcescens, inokwanisa kukanganisa DNA kana RNA, ingave yakapetwa kaviri kana imwe chete yakasungwa, mutsara kana denderedzwa pasi pemamiriro akasiyana-siyana, inoderedza zvachose nucleic acids kuita 5'-monophosphate oligonucleotides ne3-5base kureba. .Mushure mekugadzirwa kwemajini enjiniya, chigadzirwa chakaviriswa, chinoratidzwa, uye chakacheneswa muEscherichia coli (E. coli), iyo inoderedza kutaridzika kwesero supernatant uye cell lysate tsvakiridzo yesainzi, asi zvakare inovandudza kucheneswa uye kushanda kwetsvakiridzo yeprotein.Inogona zvakare kushandiswa mukurapa kwemajini, kucheneswa kwehutachiona, kugadzirwa kwevaccine, protein uye polysaccharide mishonga yeindasitiri seyakasarira yakasara nucleic acid yekubvisa reagent.
Product features
| CAS Nha. | 9025-65-4 |
| EC Nha. | |
| Molecular Weight | 30kDa |
| Isoelectric Point | 6.85 |
| Protein Purity | ≥99% (SDS-PEJI & SEC-HPLC) |
| Chaiyo Basa | ≥1.1×106U/mg |
| OptimumTemperature | 37°C |
| Optimum pH | 8.0 |
| ProteaseAction | negative |
| Bioburden | ＜10CFU/100,000U |
| Residual Host-cell Protein | ≤10ppm |
| Simbi inorema | ≤10ppm |
| Bacterial Endotoxin | ＜0.25EU/1000U |
| Kuchengetedza Buffer | 20mM Tris-HCl, pH 8.0, 2mM MgCl2, 20mM NaCl, 50% Glycerol |
Kuchengetedza mamiriro
≤0 ° C chekufambisa; -25~-15 °C Kuchengeta, makore maviri kushanda (kudzivisa kutonhora-kunyungudika).
Chikamu Tsanangudzo
Huwandu hwe enzyme yakashandiswa kushandura kukosha kwekunyura kwe △A260 ne1.0 mukati me30min pa37 °C, pH 8.0, yakaenzana neyakagayiwa 37μg salmon urume DNA nekucheka mu oligonucleotides, yakatsanangurwa sechinhu chinoshanda (U).
Kudzora kwehutano
Residual Host-cell Protein: ELISA kit
•Protease Zvisaririra: 250KU/mL UltraNuclease yakaitwa ne substrate ye60min, hapana chiitiko chakaonekwa.
•Bacterial Endotoxin: LAL-Muedzo, Pharmacopoeia yePeople's Republic of China Vhoriyamu 4 (2020 Edition) gel muganho bvunzo nzira.General Mitemo (1143).
•Bioburden: Pharmacopoeia yePeople's Republic of China Vhoriyamu 4 (2020 Edition)— General
Mitemo yeSterility Test (1101), PRC National Standard, GB 4789.2-2016.
•Simbi inorema:ICP-AES, HJ776-2015.
Operation
Basa reUltraNuclease rakadzivirirwa zvakanyanya apo SDS yakasungwa yaive pamusoro pe0.1% kana EDTA
concentration was over 1mM. Surfactant Triton X- 100, Tween 20 and Tween 80 had no effect pa nuclease
zvivakwa apo kusungwa kwaive pasi pe1.5%.
| Operation | Optimal Operation | Kushanda Kwakakodzera |
| Tembiricha | 37℃ | 0-45 ℃ |
| pH | 8.0-9.2 | 6.0- 11.0 |
| Mg2+ | 1-2mM | 1- 15mM |
| DTT | 0- 100mM | >100mM |
| 2-Mercaptoethanol | 0- 100mM | >100mM |
| Monovalent simbi ion (Na+, K+ nezvimwewo) | 0-20mM | 0-200mM |
| PO43- | 0-10mM | 0- 100mM |
Kushandisa uye Dosage
• Bvisa exogenous nucleic acid kubva kune vaccine zvigadzirwa, kuderedza njodzi yasara nucleic acid muchetura uye kunatsiridza chigadzirwa kuchengeteka.
• Deredza viscosity yemvura yekudyisa inokonzerwa ne nucleic acid, kupfupisa nguva yekugadzira uye kuwedzera chibereko cheprotein.
• Bvisa nucleic acid yakaputira chidimbu (utachiona, inclusion body, zvichingodaro), iyo inobatsira
kusunungurwa uye kucheneswa kwechidimbu.
| Kuedza Type | Kugadzirwa kweprotein | Virusi, Vaccine | Sero Zvinodhaka |
| Nhamba dzeMasero | 1g sero rakanyorova uremu (yakamiswa zvakare ne10ml buffer) | 1L kuvirisa liquid supernatant | 1L tsika |
| Minimum Dosage | 250U | 100U | 100U |
| Recommended Dosage | 2500U | 25000U | 5000U |
• Kurapa kwenyukireya kunogona kuvandudza kugadzirisa uye kudzorerwa kwemuenzaniso wekororamu chromatography, electrophoresis uye blotting analysis.
• Mukurapa kwemajini, nucleic acid inobviswa kuti iwane mavhairasi anonatswa adeno.
