Ultra Nuclease
Tsanangudzo
UltraNuclease inonzi genetically engineered endonuclaese yakabva kuSerratia marcescens, iyo inokwanisa kukanganisa DNA kana RNA, ingave yakapetwa kaviri kana imwe chete, yakatenderera kana denderedzwa pasi pemamiriro akasiyana-siyana, inosvibisa zvachose nucleic acids mu 5'-monophosphate oligonucleotides ine 3-5. base urefu.
Mushure mekugadzirwa kwemajini enjiniya, chigadzirwa chakaviriswa, chinoratidzwa, uye chakacheneswa muEscherichia coli (E. coli), iyo inoderedza viscosity yesero supernatant uye sero lysate mukutsvagisa kwesainzi, asi zvakare inovandudza kucheneswa uye kushanda kwetsvagiridzo yemapuroteni.Inogona zvakare kushandiswa mukurapa kwemajini, kucheneswa kwehutachiona, kugadzirwa kwevaccine, mapuroteni uye polysaccharide mishonga yeindasitiri seyakasarira yakasara nucleic acid yekubvisa reagent.
Kemikari Mamiriro
Chikamu Tsanangudzo
Huwandu hwe enzyme yakashandiswa kushandura kukosha kwekunyura kwe △A260 ne 1.0 mukati me30min pa37 °C, pH 8.0, yakaenzana neyakagayiwa 37μg salmon sperm DNA nekucheka mu oligonucleotides, yakatsanangurwa sechinhu chinoshanda.
Kushandisa uye Dosage
• Bvisa exogenous nucleic acid kubva kune vaccine zvigadzirwa, kuderedza njodzi yasara nucleic acid muchetura uye kunatsiridza kuchengetedzwa kwechigadzirwa.
• Deredza viscosity yemvura yekudya inokonzerwa ne nucleic acid, kupfupisa nguva yekugadzira uye kuwedzera chibereko cheprotein.
• Bvisa nucleic acid iyo yakaputira chidimbu (virusi, inclusion body, etc.), iyo inobatsira kusunungurwa uye kucheneswa kwechidimbu.
• Kurapa kwenyukireya kunogona kuvandudza kugadziriswa uye kudzorerwa kwemuenzaniso wekororamu chromatography, electrophoresis uye blotting analysis.
• Mukurapa kwemajini, nucleic acid inobviswa kuti iwane mavhairasi anonatswa adeno.
Tsanangudzo
| Test Items | Zvinotsanangurwa |
| Tsanangudzo | Zvakajeka uye Zvisina Ruvara |
| Chiitiko | ≥ 250 U/ul |
| Chiitiko chakananga | ≥1.1*106U/mg |
| Kuchena (SDS-PEJI) | ≥ 99.0% |
| MaProteases | Hapana chaonekwa |
| Bioburden | ＜10 cfu/100,000U |
| Endotoxines(LAL-bvunzo) | ＜ 0.25EU/1,000U |
Kutakura uye kuchengetedza
Zvekufambisa:Inotumirwa pasi pe0 °C
Kuchengeta:Chengetedza pa -25~-15°C
Inokurudzirwa edzazve Hupenyu:2 gore (kudzivisa kutonhora-kunyorova)
