RT-LAMP Colormetric (Lyophilized bhora)

Ichi chigadzirwa chine reaction buffer, RT-Enzymes Mix (Bst DNA polymerase uye inodzivirira kupisa reverse transcriptase), zvinodzivirira lyophilized uye chromogenic dhayi zvikamu.Chigadzirwa chacho chiri lyophilized bhora mhando, uchishandisa chete neprimers uye matemplate.Iyi kit inopa kukurumidza, kwakajeka kwekuona kuonekwa kwekusimudzira, iyo yakaipa inoratidzira inoratidzwa mutsvuku uye maitiro akanaka inoratidzwa nekuchinja kune yellow.

Chikamu

RT-LAMP Colormetric Master Mix (Lyophilized beads)

Applications

YeDNA kana RNA isothermal amplification.

Storage Conditions

Kutakurwa uye kuchengetwa pa2 ~ 8 ℃.Chigadzirwa chacho chinoshanda kwemwedzi gumi nemiviri.

Protocol

1.Bvisa nhamba inoenderana Lyophilized beads poda zvichienderana nehuwandu hwebvunzo.

2.Gadzirira reaction mix

Notes:

1. 10 × Primer Mix Concentration: 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Loop F / B;

2. Nucleic acid templates inokurudzirwa kuti iparadzwe uchishandisa DEPC mvura.

3.Isa pa 65 ° C kwe 30-45mins, iyo inogona kuwedzerwa zvakakodzera maererano nekuchinja kwemavara Reaction time.

4.Sekuona ziso, yero yaive yakanaka uye tsvuku yaive negative.

Notes

1.Iyo tembiricha yekuita inogona kugadziridzwa pakati pe62 ℃ uye 68 ℃ zvichienderana neiyo primer mamiriro.

2.Izvo zvakaputirwa reagents hazvifanirwe kuratidzwa kumhepo kwenguva yakareba.

3.Iyo tsvuku uye yero discoloration reaction inotsamira pane pH shanduko yemaitiro system, ndapota usashandise ine Tris nucleic acid yekuchengetedza mhinduro, inokurudzirwa kushandisa ddH.₂O yakachengetwa nucleic acid.

4.Kuedza kuchaitwa nenzira yakarongeka, kusanganisira kugadzirira kwemaitiro ekugadzirisa, kurapwa kwemuenzaniso uye kuwedzera kwemuenzaniso.

5.Zvinokurudzirwa kugadzirira reaction system muiyo yekupedzisira-yakachena tafura uye kuwedzera matemplate mufume hood yemamwe makamuri kudzivirira nhema.