Ribonuclease III (RNase III)
Tsanangudzo
Ichi chigadzirwa chinonzi ribonuclease III (RNase III) chinoratidzwa zvakare neE. coli.Iyi chaiyo exonuclease inotsemura RNA yakapetwa kaviri (dsRNA) uye inogadzira 12-35bp dsRNA zvimedu zvine 5'-PO4 uye 3'-OH, 3' overhangs.
Kemikari Mamiriro
Chikamu Tsanangudzo
Chiitiko cheyuniti tsananguro: Chikamu chimwe chinoreva huwandu hweenzayimu inodiwa kuderedza 1 μg ye
dsRNA kune siRNA mune 50 μL reaction system pa37°C kwemaminetsi makumi maviri.
Tsanangudzo
Test Items | Zvinotsanangurwa |
Exonuclease Chiitiko | Inoburitswa< 0.1% yeiyo yakazara radioactivity |
Non-Specific Dnase Chiitiko | Haioneki |
Protein Purity Assay (SDS-PEJI) | ≥ 95% |
RNase Chiitiko (Yakawedzerwa Digestion) | >90% ye substrate RNA inoramba yakasimba |
Kutakura uye kuchengetedza
Zvekufambisa:Dry ice
Kuchengeta:Chengetedza pa -25~-15°C
Inokurudzirwa edzazve Hupenyu:2 gore
zvinoenderana zvigadzirwa
Nyora meseji yako pano uye titumire kwatiri