Recombinant Deoxyribonuclease I (DNase I, RNase-isina)
Tsanangudzo
Recombinant Deoxyribonuclease I, is endonuclease inokwanisa kugaya DNA ine tambo imwe chete kana kaviri.Iyo hydrolyzes phosphodiester zvisungo kugadzira mono- uye oligodeoxynucleotides ine 5'-phosphate mapoka uye 3'-OH mapoka.Iyo yakakwana yekushanda pH huwandu hweDNase I ndeye 7-8.Basa reDNase I rinoenderana neCa2 + uye rinogona kushandiswa ne divalent metal ions yakadai seCo2 +, Mn2 +, Zn2 +, etc. Pamberi peMg2 +, DNase ndinogona kutema DNA yakapetwa kaviri pane chero nzvimbo;apo pamberi peMn2 +, DNase ndinogona kucheka DNA yakapetwa kaviri panzvimbo imwe chete, kuumba magumo asina kujeka kana 1-2 nuclei Sticky inopera ine nucleotide overhangs.Enzayemu iyi inotorwa kubva kunezve E. coli strains, haina RNase, uye inogona kushandiswa kugadzira akasiyana siyana eRNA samples.
Chimiro chemakemikari
Applications
RNA kuburitsa: gadzirira RNA isina DNA;
Mushure me in vitro transcription neRNA polymerase, yakadai seT7 RNA Polymerase (Katsi#10618), inoshandiswa kubvisa template DNA;
Gadzirira DNA-isina RNA pamberi peRT-PCR uye RT-qPCR;
Inoshandiswa pamwe chete neDNA Polymerase I (Katsi#12903) pakunyora mazita eDNA neshanduro yechinic;
Inoshandiswa mukuongorora kwetsoka kuongorora DNA-protein kuwirirana;
Gadzira raibhurari yezvimedu zvisina tsarukano;
Mukuonekwa kweTUNEL yeapoptosis, iyo genomic DNA yakaveurwa zvishoma yakashandiswa sekutonga kwakanaka.
Kutumira uye Kuchengeta
Zvekufambisa:Ice packs
Storage Conditions:Chengetedza mu -20 ℃
Sherufu Hupenyu:2 years
Notes:
1.Enzymes inofanira kuchengetwa mubhokisi rechando kana pabhati yechando kana yakashandiswa, uye inofanira kuchengetwa pa -20 ° C pakarepo mushure mekushandisa.
2.Nekuchengetedza kwako uye hutano, ndapota pfeka midziyo yekudzivirira yega (PPE), yakadai sejasi re laboratori uye magirovhosi anoraswa, paunenge uchishanda nechigadzirwa ichi.
