M-MLV Reverse Transcriptase (Glycerol yemahara)
A lyophilizable Reverse Transcriptase.Inogona kuiswa kune yakadzika lyophilization tekinoroji uchichengetedza yakakura reverse transcription kuita uye kugadzikana.Ichi chigadzirwa hachina excipients, ndapota wedzera chako sezvinodiwa.
Zvikamu
Chikamu | HC2005A-01 (10,000U) | HC2005A-02 (40,000U) |
Reverse Transcriptase (Glycerol Yemahara) (200U/μL) | 50 μL | 200 μL |
5 × Buffer | 200 μL | 800 μL |
Application:
Inoshanda kune imwe-nhanho RT-qPCR maitiro.
Storage Condition
Chengetedza pa -30 ~ -15°C uye chotakura pa ≤0°C.
Chikamu Tsanangudzo
Imwe unit (U) inotsanangurwa sehuwandu hwe enzyme inobatanidza 1 nmol ye dTTP mu acid-insoluble material mu 10mins pa 37 ° C, ne Poly(rA)·Oligo (dT) se template / primer.
Notes
Yekushandisa tsvakurudzo chete.Kwete kushandiswa mukuongorora maitiro.
1.Ndapota chengeta nzvimbo yekuedza yakachena;Pfeka magirovhosi anoraswa nemasiki;Shandisa RNase-isina consumables senge centrifuge chubhu uye pipette mazano.
2.Chengetedza RNA paaizi kudzivirira kudzikira.
3.Yemhando yepamusoro RNA templates inokurudzirwa kuti iwane yakakwira kunyatsoshanda reverse transcription.