DNase I
DNase I (Deoxyribonuclease I) inonzi endodeoxyribonuclease inokwanisa kugaya DNA imwe kana ine tambo mbiri.Inoziva uye inotsemura phosphodiester zvisungo kugadzira monodeoxynucleotides kana imwe- kana kaviri-yakasungwa oligodeoxynucleotides nemapoka ephosphate pa5'-terminal uye hydroxyl pa3'-terminal.Chiitiko cheDNase I chinotsamira paCa2 + uye chinogona kuitwa ne divalent simbi ions seMn2 + uye Zn2 +.5mM Ca2 + inodzivirira iyo enzyme kubva kune hydrolysis.Iine Mg2+, iyo enzyme yaigona kuona nekutsemura chero nzvimbo pane chero tambo yeDNA.Muhupo hweMn2+, tambo mbiri dzeDNA dzinogona kuzivikanwa panguva imwe chete uye dzakanamirwa panenge pasaiti imwe chete kuti iumbe magumo eDNA zvimedu kana anonamira magumo eDNA zvimedu zvine 1-2 nucleotides inobuda.
Product Property
Bovine Pancreas DNase Ini ndakaratidzwa mune mbiriso yekutaura system uye ndakacheneswa.
Cvanopikisa
| Chikamu | Volume | |||
| 0.1KU | 1KU | 5KU | 50KU | |
| DNase I, RNase-isina | 20μL | 200μL | 1mL | 10mL |
| 10 × DNase I Buffer | 1mL | 1mL | 5 × 1mL | 5 × 10mL |
Kutakura uye Kuchengeta
1. Kuchengetedza Kugadzikana: - 15 ℃~-25 ℃ yekuchengetedza;
2.Transport Kugadzikana: Kutakura pasi pechando packs;
3. Inowanikwa mu: 10 mM Tris-HCl, 2 mM CaCl2, 50% glycerol, pH 7.6 pa25 ℃.
Chikamu Tsanangudzo
Chimwe chikamu chinotsanangurwa sehuwandu hwe enzyme inozosvibisa zvachose 1 µg yepBR322 DNA mumaminetsi gumi pa37°C.
Quality Control
RNase:5U yeDNase I ine 1.6 μg MS2 RNA kwemaawa mana pa37 ℃ haibudisi kushatisa sekutemerwa neagarose gel electrophoresis.
Utachiona Endotoxin:LAL-yedzo, maererano neChinese Pharmacopoeia IV 2020 edition, gel muganho bvunzo nzira, general mutemo (1143).Bacterial endotoxin content inofanira kuva ≤10 EU/mg.
Mirayiridzo Yekushandisa
1.Gadzirira mhinduro yemhinduro muRNase-yemahara chubhu zvinoenderana nezviyero zvakanyorwa pazasi:
| Chikamu | Volume |
| RNA | X μg |
| 10 × DNase I Buffer | 1 μL |
| DNase I, RNase-isina(5U/μL) | 1 U pa μg RNA |
| ddH2O | Kusvika ku10 μL |
2.37 ℃ kwemaminitsi gumi nemashanu;
3.Wedzera buffer yekugumisa kuti umise maitiro, uye kupisa pa 65 ℃ kwemaminetsi e10 kuti usashandise DNase I. Muenzaniso unogona kushandiswa zvakananga kune chinotevera chinyorwa chekuedza.
Notes
1.Shandisa 1U DNase I pa μg yeRNA, kana 1U DNase I isingasviki 1μg yeRNA.
2.EDTA inofanira kuwedzerwa kune imwe yekupedzisira ye5 mM kuchengetedza RNA kubva pakusvibiswa panguva yekushanda kwe enzyme.
