Poly A Inotakura RNA
Katsi Nhamba: HC4001A
Poly A, polyadenylate, musanganiswa we100 ~ 10000 polyadenylates, iyo inogadzirwa ne polynucleotide phosphorylase in vitro.Mu vivo, poly (a) inowedzerwa kune 3-terminal ye mRNA ne enzyme kuvandudza kugadzikana kwemRNA.Mukushandiswa kweiyo nucleic acid yekubvisa, kuwedzera poly A kune lysate kana inosunga mhinduro inogona kuvandudza goho reDNA neRNA.The mechanismyepoly A kuvandudza goho re nucleic acid sezvizvi:
1. Saturated kuonana nepamusoro adsorption yezvinyorwa.Zvizhinji zve polypropylene zvinyorwa zvine static magetsi pamusoro, izvo zvinozove adsorb nucleic acids.Inotakura RNA inogona kugutsa izviadsorption mhedzisiro uye kuderedza kurasikirwa kwechinangwa nucleic acids.
2. Ita kuti trace nuclease isashande: Kune manuclease akasiyana mubiological samples uyezvakatipoteredza.Poly A inogona kumisa trace nucleases mukutora kana kuchengetedza matanho kutikunatsiridza goho uye kugadzikana kwechinangwa nucleic acids.
3. Kunaya: Munhanho yekuchenesa nucleic acid yedoro yakapindirana kunaya kana kusunga, poly A inogona kupindirana neinongedzo nucleic acid kana kuumba polymer particleskunatsiridza kupora.
Kuchengetedza mamiriro
-20 ~ 8 ℃, yakaoma kuchengetedza, kuchengetedza kwenguva refu kunofanirwa kuiswa pa -20 ℃
Tsanangudzo
| Nhamba yeCAS | 26763-19-9 |
| Chitarisiko | White lyophilized powder |
| Kuchena | 99% |
| Molecular Weight | 700-3500 KDa |
Kushandisa nzira
Tora huwandu hwakakodzera hweiyo lyophilized poda, wedzera DEPC yakabikwa mvura kana guanidine munyu mhinduronyungudutsa mu 0.1-1ug/uL, wobva waiisa pasi woichengeta pa -20°C.
Applications
1.Virus DNA / RNA extraction: kuwedzera 1-5ug Carrier RNA kune lysate inogona kuvandudza goho reRNA / DNA, kudzikamisa chinangwa nucleic acid uye kudzivisa kusvibiswa kwecheneswa nucleic acid panguva yekuchengetedza.
2. Mu micro DNA / RNA extraction by column membrane method (<1ug), kuwedzera mutakuri weRNA ku1-5ug inobatsira kuvandudza goho re nucleic acid.
3. Muchinwiwa chinodhaka chinonzi nucleic acid precipitation uye danho rekufungisisa, kuwedzera kwe1-2ug mutakuri weRNA kunobatsira kuvandudza kudzorerwa kwechikamu chiduku cheRNA.
4. Muhuwandu hwekuongorora PCR reaction solution, kuwedzera 10-100ng carrier RNA kune mhinduro yekugadzirisa inobatsira kuvandudza kunzwisisika uye kuderedza C.Tvalue.
