Imwe Nhanho RT-qPCR probe kit
Tsanangudzo
Imwe Danho qRT-PCR Probe Kit yakanyatsogadzirirwa qPCR inoshandisa zvakananga RNA (eg virus RNA) setemplate.Uchishandisa gene specific primers (GSP), iyo reverse transcription uye qPCR inogona kupedzwa muchubhu imwe, ichideredza zvakanyanya maipiping maitiro uye njodzi yekusvibiswa.Inogona kuvharwa pa55 ℃ isingakanganise kushanda uye kunzwa kweqRT-PCR.Imwe Nhanho qRT-PCR Probe Kit inopihwa muMaster Mix.Iyo 5 × One Danho Musanganiswa ine optimized buffer uye dNTP/dUTP Musanganiswa, uye yakakodzera kune yakakwirira-specificity yekuona masisitimu zvichienderana nefluorescence yakanyorwa probes (eg TaqMan).
Nheyo dzekutanga dzeRT-qPCR
Tsanangudzo
| Test Items | Tsanangudzo | Mhedzisiro |
| (SDS PEJI) Kuchena kweEnzyme Stock (SDS PEJI) | ≥95% | Pass |
| Endonuclease Chiitiko | Haisati yaonekwa | Pass |
| Exodulease Chiitiko | Haisati yaonekwa | Pass |
| Rnase Chiitiko | Haisati yaonekwa | Pass |
| Residual E.coli DNA | ＜1makopi/60 | Pass |
| Functional Assay-System | 90%≤110% | Pass |
Zvikamu
| Zvikamu | 100rxns | 1,000rns | 5,000 rxns |
| RNase-yemahara ddH2O | 2*1ml | 20ml | 100ml |
| 5 * imwe nhanho musanganiswa | 600μl | 6*1ml | 30ml |
| Imwe nhanho enzyme musanganiswa | 150μl | 2*750μl | 7.5ml |
| 50* ROX referenzi Dye 1 | 60μl | 600μl | 3*1ml |
| 50* ROX referensi Dye 2 | 60μl | 600μl | 3*1ml |
a.Imwe-Nhanho Buffer inosanganisira dNTP Musanganiswa uye Mg2+.
b.Enzyme Mix inonyanya kuve nereverse
transcriptase, Kupisa Kutanga Taq DNA polymerase (antibody modification) uye RNase inhibitor.
c.Inoshandiswa kugadzirisa kukanganisa kwefluorescene sinal pakati pematsime akasiyana.
d.ROX: Iwe unofanirwa kusarudza iyo calibration zvinoenderana nemhando yechiridzwa chekuyedza.
Applications
Kuonekwa kweQPCR
Kutumira uye Kuchengeta
KutumiraIce packs
Storage Conditions:Chengetedza pa -20 ℃.
Shief Hupenyu:18months
