Glucose Dehydrogenase (GDH)
Tsanangudzo
Glutamate Dehydrogenase (GDH) inonzi mitochondrial enzyme inokonzera reversible oxidative deamination ye glutamate kune a-ketoglutarate uye inoshanda sechinhu chinokosha pakati pevanabolic uye catabolic nzira.Mumhuka dzinoyamwisa, GDH inoenderana neallosteric regulation uye ine basa rakakura muchiropa, itsvo, uropi, uye pancreas.Basa reGDH muserum rinogona kushandiswa kusiyanisa zvirwere zvechiropa nekuda kwekuputika kwechiropa, izvo zvisingaratidzi serum yakakwirira yeGDH basa, uye zvirwere zvinoguma nehepatocyte necrosis, iyo inoguma nepamusoro serum GDH.
Chiitiko cheGDH chinotemerwa neyakasanganiswa enzyme assay umo glutamate inodyiwa neGDH inogadzira NADH, iyo inopindirana neprobe inogadzira colorimetric (450 nm) chigadzirwa zvinoenderana nechiitiko cheGDH chiripo.Imwe yuniti yeGDH huwandu hweenzyme inoburitsa 1.0 mmole yeNADH paminiti papH 7.6 pa37 °C.
Mushonga Wemakemikari
Reaction mechanism
D-Glucose + mugamuchiri → D-Glucono-1,5-lactone + yakaderedzwa kugamuchira
Tsanangudzo
| Test Items | Zvinotsanangurwa |
| Tsanangudzo | White amorphous powder, lyophilized |
| Chiitiko | ≥160U/mg |
| Kuchena(SDS-PEJI) | ≥90% |
| Solubility (10 mg poda/mL) | Pachena |
| Kusvibisa ma enzymes | |
| Glucose dehydrogenase (NAD) | ≤0.02% |
| Hexokinase | ≤0.02% |
| A-Glucosidase | ≤0.02% |
Kutakura uye kuchengetedza
Transportation: Ice packs
Storage :Chengetedza pa -25~-15°C(Nguva refu), 2-8°C (nguva pfupi)
Inokurudzirwa kuongorora zvakareHupenyu: 18 mwedzi
