CHO HCP ELISA Kit
Imwe-nhanho immunosorbent ELISA nzira inoshandiswa mune iyi bvunzo.Samples ine CHOK1 HCP panguva imwe chete inopindirana neHRP-yakanyorwa mbudzi anti-CHOK1 antibody uye anti-CHOK1 antibody yakavharwa paELISA ndiro, pakupedzisira kugadzira sandwich complex ye solid-phase antibody-HCP-yakanyorwa antibody.Antigen-antibody isina kusungwa inogona kubviswa nekugeza ndiro yeELISA.Iyo TMB substrate inowedzerwa kutsime kuitira kukwana kwakakwana.Iko kukura kwemavara kunomiswa mushure mekuwedzera mhinduro yekumisa, uye iyo OD kana kukosha kwekutora kwemhinduro mhinduro pa450/650nm inoverengwa ne microplate reader.Iko kukosha kweOD kana kukosha kwekunyura kwakaenzana kune HCP zvirimo mumhinduro.Kubva pane izvi, huwandu hweHCP mune mhinduro inogona kuverengerwa zvinoenderana neyakajairwa curve.
Application
Iyi kit inoshandiswa kuona huwandu hweCHOK1 host cell protein yasara mumasampuli.
Cvanopikisa
| S/N | Chikamu | Concentration | Storage Conditions |
| 1 | CHOK1 HCP Standard | 0.5mg/mL | ≤–20℃ |
| 2 | Anti-CHO HCP-HRP | 0.5mg/mL | ≤–20℃, chengetedza kubva kuchiedza |
| 3 | TMB | NA | 2-8 ℃, chengetedza kubva kuchiedza |
| 4 | 20 × PBST 0.05% | NA | 2-8℃ |
| 5 | Stop solution | NA | RT |
| 6 | Microplate sealers | NA | RT |
| 7 | BSA | NA | 2-8℃ |
| 8 | High adsorption pre-coating Plates | NA | 2-8℃ |
Equipment Inodiwa
| Consumables / Equipment | Kugadzira | Catalog |
| Microplate reader | Molecular Devices | Spectra Max M5, M5e, kana yakaenzana |
| Thermomixer | Eppendorf | Eppendorf/5355, kana zvakaenzana |
| Vortex musanganisi | IKA | MS3 Digital, kana yakaenzana |
Kuchengetedza uye kugadzikana
1.Kutakura pa -25~-15°C.
2.Mamiriro ekuchengetedza anoratidzwa muTebhura 1;zvikamu 1-2 zvakachengetwa ≤-20 ° C, 5-6 zvakachengetwa RT, 3,4, 7, 8 inochengetwa pa 2-8 ℃;nguva yekushanda ndeye 12 mwedzi.
Product parameters
1.Sensitivity: 1ng/mL
2.Kuonekwa kwemhando: 3- 100ng / mL
3.Kunyatsojeka: Intra-assay CV≤ 10%, inter-assay CV≤ 15%
4.HCP kuvharika:> 80%
5.Tsanangudzo: Kiti iyi ndeyepasirese sezvo ichinyatsoita neCHOK1 HCP yakazvimirira yekuchenesa maitiro.
Reagent kugadzirira
1.PBST 0.05%
Tora 15 ml ye20 × PBST 0.05%, yakaderedzwa muddH2O, uye yakagadzirwa kusvika ku300 ml.
2.1.0% BSA
Tora 1g yeBSA kubva mubhodhoro uye wedzera mu100 ml yePBST 0.05%, sanganisa zvakanaka kusvika yanyungudika zvachose, uye chengeta pa2-8°C.Iyo yakagadzirirwa dilution buffer inoshanda kwemazuva manomwe.Inokurudzirwa kugadzirira sezvinodiwa.
3.Mhinduro yekuona 2μg/mL
Tora 48μL ye 0.5 mg/mL Anti-CHO HCP-HRP uye dilute mu 11,952μL ye 1% BSA kuti uwane yekupedzisira kusanganiswa kwe 2μg/mL yekuona mhinduro.
4.QC uye Kugadzirira kweCHOK1 HCP Maitiro
| Tube Aihwa. | Original | Concentration | Volume | 1%BSA | Vhoriyamu yose | Final |
| A | Standard | 0.5mg/mL | 10 | 490 | 500 | 10,000 |
| B | A | 10,000 | 50 | 450 | 500 | 1,000 |
| S1 | B | 1.000 | 50 | 450 | 500 | 100 |
| S2 | S1 | 100 | 300 | 100 | 400 | 75 |
| S3 | S2 | 75 | 200 | 175 | 375 | 40 |
| S4 | S3 | 40 | 150 | 350 | 500 | 12 |
| S5 | S4 | 12 | 200 | 200 | 400 | 6 |
| S6 | S5 | 6 | 200 | 200 | 400 | 3 |
| NC | NA | NA | NA | 200 | 200 | 0 |
| QC | S1 | 100 | 50 | 200 | 250 | 20 |
Tafura: Kugadzirira kweQC uye Maitiro
Assay Procedure
1.Gadzirira ma reagents sezvakaratidzwa mu "Reagent Preparation" pamusoro.
2.Tora 50μL yezviyero, samples uye QCs (tarisa Tafura 3) mutsime rega rega, wobva wawedzera 100μL yeDetection solution (2μg/mL);Dhavhara ndiro nechisimbiso, uye isa ndiro yeELISA pane thermomixer.Bata pa500rpm, 25±3℃ kwemaawa maviri.
3.Pindura iyo microplate mudhishi uye urase mhinduro yekuputira.Pipette 300μL yePBST 0.05% mugomba rega rega kuti ugeze ndiro yeELISA uye kurasa mhinduro, uye dzokorora kugeza katatu.Pindura ndiro pajira rakachena uye woomesa.
4.Wedzera 100μL yeTMB substrate (ona Tafura 1) kune imwe neimwe tsime, simbisa ndiro yeELISA, uye incuite murima pa25±3℃ kwemaminetsi gumi nemashanu.
5.Pipette 100μL yekumisa mhinduro mutsime rega rega.
6.Yera kunyura pawavelength ye450/650nm ine microplate muverengi.
7.Ongorora data neSoftMax kana software yakafanana.Ronga mwero curve uchishandisa mana-parameta logistic regression modhi.
Standard Curve Muenzaniso
CHERECHEDZA: Kana iyo yekumisikidzwa kweHCP mumuenzaniso yakadarika muganho wepamusoro weiyo yakajairwa curve, inoda kucheneswa nemazvo nedilution buffer isati yaedzwa.
MAZANO
Iyo yekumisa mhinduro ndeye 2M sulfuric acid, ndapota bata nekuchenjerera kuti usaputika!
